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. 2007 Dec;9(12):1099–1110. doi: 10.1593/neo.07721

Figure 5.

Figure 5

Lysosomal localization of mAb 806-Cy3 in A431 Cells. (A) Preincubated mAb 806-Cy3 (red) was allowed to internalize for 60 minutes at 37°C. Cells were subsequently fixed and stained for LAMP1 (green). Colocalization is indicated by arrows in the merged images. Scale bar, 20 µm. (B) Time-lapse microscopy of internalization and colocalization of mAb 806-Cy3 (red) and lgp-120-GFP (green) in A341 cells. Sequential images of transfected A431 cells are shown at 0, 30, 45, and 60 minutes. Colocalization is indicated by the arrows. Scale bar, 20 µm. Refer to Supplemental data for Video W4.