Abstract
It has been shown experimentally that mixtures of two types of particles, namely, elementary bodies of vaccinia and collodion particles coated with protein, sediment with a single boundary in the analytical centrifuge. Such mixtures have been shown to develop one or two boundaries on electrophoresis in the Tiselius apparatus, depending on the type of coating on the surface of the collodion particles. When covered with the heat-stable soluble antigen of vaccinia, collodion particles migrate in the electrical field at the same rate as elementary bodies. On the other hand, if they are coated with a component of normal rabbit serum, they migrate at a different rate. The estimation of purity of preparations of virus by means of data obtained by ultracentrifugation and electrophoresis is discussed.
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Selected References
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- Bauer J. H., Pickels E. G. AN IMPROVED AIR-DRIVEN TYPE OF ULTRACENTRIFUGE FOR MOLECULAR SEDIMENTATION. J Exp Med. 1937 Mar 31;65(4):565–586. doi: 10.1084/jem.65.4.565. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gates F. L. A METHOD OF STANDARDIZING BACTERIAL SUSPENSIONS. J Exp Med. 1920 Jan 1;31(1):105–114. doi: 10.1084/jem.31.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pickels E. G., Bauer J. H. ULTRACENTRIFUGATION STUDIES OF YELLOW FEVER VIRUS. J Exp Med. 1940 Apr 30;71(5):703–717. doi: 10.1084/jem.71.5.703. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shedlovsky T., Smadel J. E. ELECTROPHORETIC STUDIES ON ELEMENTARY BODIES OF VACCINIA. J Exp Med. 1940 Oct 31;72(5):511–521. doi: 10.1084/jem.72.5.511. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smadel J. E., Pickels E. G., Shedlovsky T. ULTRACENTRIFUGATION STUDIES ON THE ELEMENTARY BODIES OF VACCINE VIRUS : II. THE INFLUENCE OF SUCROSE, GLYCEROL, AND UREA SOLUTIONS ON THE PHYSICAL NATURE OF VACCINE VIRUS. J Exp Med. 1938 Sep 30;68(4):607–627. doi: 10.1084/jem.68.4.607. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smadel J. E., Rivers T. M., Pickels E. G. ESTIMATION OF THE PURITY OF PREPARATIONS OF ELEMENTARY BODIES OF VACCINIA. J Exp Med. 1939 Sep 30;70(4):379–385. doi: 10.1084/jem.70.4.379. [DOI] [PMC free article] [PubMed] [Google Scholar]