Abstract
It has been possible by means of classical chemical methods to isolate and to characterize to some extent the nucleic acid of elementary bodies of vaccinia. Determination by means of diphenylamine reagent revealed that the major part of the nucleic acid was of the thymus type. This was further substantiated by its stability in the presence of ribonuclease, less than 10 per cent undergoing depolymerization during prolonged incubation at 37°C. By the technique employed, at least 5.6 per cent of the virus was shown to be thymonucleic acid. This amount agreed favorably with the value calculated from the non-lipid organic phosphorus of elementary bodies on the assumption that the phosphorus bound in the organic form was derived principally from nucleic acid.
Full Text
The Full Text of this article is available as a PDF (458.6 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Claude A. CONCENTRATION AND PURIFICATION OF CHICKEN TUMOR I AGENT. Science. 1938 May 20;87(2264):467–468. doi: 10.1126/science.87.2264.467. [DOI] [PubMed] [Google Scholar]
- Hoagland C. L., Smadel J. E., Rivers T. M. CONSTITUENTS OF ELEMENTARY BODIES OF VACCINIA : I. CERTAIN BASIC ANALYSES AND OBSERVATIONS ON LIPID COMPONENTS OF THE VIRUS. J Exp Med. 1940 May 31;71(6):737–750. doi: 10.1084/jem.71.6.737. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kunitz M. ISOLATION FROM BEEF PANCREAS OF A CRYSTALLINE PROTEIN POSSESSING RIBONUCLEASE ACTIVITY. Science. 1939 Aug 4;90(2327):112–113. doi: 10.1126/science.90.2327.112. [DOI] [PubMed] [Google Scholar]
- Smadel J. E., Lavin G. I., Dubos R. J. SOME CONSTITUENTS OF ELEMENTARY BODIES OF VACCINIA. J Exp Med. 1940 Feb 29;71(3):373–389. doi: 10.1084/jem.71.3.373. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smadel J. E., Rivers T. M., Pickels E. G. ESTIMATION OF THE PURITY OF PREPARATIONS OF ELEMENTARY BODIES OF VACCINIA. J Exp Med. 1939 Sep 30;70(4):379–385. doi: 10.1084/jem.70.4.379. [DOI] [PMC free article] [PubMed] [Google Scholar]