Abstract
1. In two rabbits subjected to prolonged injections with crystalline egg albumin the antibodies in one showed progressive changes such as noted in an earlier paper; the antibodies in the other did not. 2. The significance of this behavior in the production of sera for therapeutic use is pointed out. 3. Quantitative studies are reported on the low grade or incomplete antibody present in the early stages of immunization of a horse with egg albumin. 4. Quantitative studies on the flocculating antibody from later bleedings from the horse are given, and the dissociation of the soluble pre-zone compounds by rabbit anti-egg albumin is studied. Rough velocity estimations are reported. 5. The bearing of the findings on the mechanism of precipitin and flocculation reactions and of the Danysz effect is discussed in terms of the union of multivalent antigen with multivalent antibody.
Full Text
The Full Text of this article is available as a PDF (646.5 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Heidelberger M., Kabat E. A. CHEMICAL STUDIES ON BACTERIAL AGGLUTINATION : III. A REACTION MECHANISM AND A QUANTITATIVE THEORY. J Exp Med. 1937 May 31;65(6):885–902. doi: 10.1084/jem.65.6.885. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heidelberger M., Kendall F. E. A QUANTITATIVE THEORY OF THE PRECIPITIN REACTION : II. A STUDY OF AN AZOPROTEIN-ANTIBODY SYSTEM. J Exp Med. 1935 Sep 30;62(4):467–483. doi: 10.1084/jem.62.4.467. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heidelberger M., Kendall F. E. A QUANTITATIVE THEORY OF THE PRECIPITIN REACTION : IV. THE REACTION OF PNEUMOCOCCUS SPECIFIC POLYSACCHARIDES WITH HOMOLOGOUS RABBIT ANTISERA. J Exp Med. 1937 Apr 30;65(5):647–660. doi: 10.1084/jem.65.5.647. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heidelberger M., Kendall F. E. THE PRECIPITIN REACTION BETWEEN TYPE III PNEUMOCOCCUS POLYSACCHARIDE AND HOMOLOGOUS ANTIBODY : II. CONDITIONS FOR QUANTITATIVE PRECIPITATION OF ANTIBODY IN HORSE SERA. J Exp Med. 1935 Mar 31;61(4):559–562. doi: 10.1084/jem.61.4.559. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kabat E. A., Heidelberger M. A QUANTITATIVE THEORY OF THE PRECIPITIN REACTION : V. THE REACTION BETWEEN CRYSTALLINE HORSE SERUM ALBUMIN AND ANTIBODY FORMED IN THE RABBIT. J Exp Med. 1937 Jul 31;66(2):229–250. doi: 10.1084/jem.66.2.229. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pappenheimer A. M. ANTI-EGG ALBUMIN ANTIBODY IN THE HORSE. J Exp Med. 1940 Jan 31;71(2):263–269. doi: 10.1084/jem.71.2.263. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sabin F. R. CELLULAR REACTIONS TO A DYE-PROTEIN WITH A CONCEPT OF THE MECHANISM OF ANTIBODY FORMATION. J Exp Med. 1939 Jun 30;70(1):67–82. doi: 10.1084/jem.70.1.67. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stokinger H. E., Heidelberger M. A QUANTITATIVE THEORY OF THE PRECIPITIN REACTION : VI. THE REACTION BETWEEN MAMMALIAN THYROGLOBULINS AND ANTIBODIES TO HOMOLOGOUS AND HETEROLOGOUS PREPARATIONS. J Exp Med. 1937 Jul 31;66(2):251–272. doi: 10.1084/jem.66.2.251. [DOI] [PMC free article] [PubMed] [Google Scholar]