Abstract
A comparative study of spreading factor and hyaluronidase in preparations from various sources revealed the following points of similarity and dissimilarity in the two reactions. 1. Similarities: (a) All preparations containing hyaluronidase also produced spreading. (b) Heating at 65° and 100°C. for 30 minutes produced a comparable effect on both reactions. (c) The demonstration of the presence of hyaluronic acid in skin offers a plausible explanation for the mechanism of spreading on the basis of hyaluronidase activity. 2. Dissimilarities: (a) No parallelism was observed in the degree of activity of spreading factor and hyaluronidase in the same preparations. (b) All preparations which produced spreading did not contain hyaluronidase. (c) Antisera to hyaluronidase preparations specifically and completely inhibited the activity of the homologous enzyme but did not inhibit the spreading factor in the same preparations. The significance of the similarities and dissimilarities between the two reactions is discussed. It is concluded that while hyaluronidase may play a rôle in the spreading reaction the phenomenon is a complex one and cannot be explained on the basis of a simple chemical reaction.
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Selected References
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- Claude A. SPREADING PROPERTIES OF LEECH EXTRACTS AND THE FORMATION OF LYMPH. J Exp Med. 1937 Aug 31;66(3):353–366. doi: 10.1084/jem.66.3.353. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Claude A. THE ENHANCING EFFECT OF AZOPROTEINS ON THE LESIONS PRODUCED BY VACCINE VIRUS, THE SHOPE FIBROMA VIRUS, AND THE AGENT TRANSMITTING CHICKEN TUMOR I. J Exp Med. 1939 Apr 30;69(5):641–648. doi: 10.1084/jem.69.5.641. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Duran-Reynals F. STUDIES ON A CERTAIN SPREADING FACTOR EXISTING IN BACTERIA AND ITS SIGNIFICANCE FOR BACTERIAL INVASIVENESS. J Exp Med. 1933 Jul 31;58(2):161–181. doi: 10.1084/jem.58.2.161. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Duran-Reynals F. STUDIES ON A CERTAIN SPREADING FACTOR EXISTING IN BACTERIA AND ITS SIGNIFICANCE FOR BACTERIAL INVASIVENESS. J Exp Med. 1933 Jul 31;58(2):161–181. doi: 10.1084/jem.58.2.161. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Duran-Reynals F. THE EFFECT OF ANTITESTICULAR SERUM ON THE ENHANCEMENT VALUE OF TESTICLE EXTRACT. J Exp Med. 1932 Apr 30;55(5):703–712. doi: 10.1084/jem.55.5.703. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Duran-Reynals F. THE EFFECT OF EXTRACTS OF CERTAIN ORGANS FROM NORMAL AND IMMUNIZED ANIMALS ON THE INFECTING POWER OF VACCINE VIRUS. J Exp Med. 1929 Aug 31;50(3):327–340. doi: 10.1084/jem.50.3.327. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hoffman D. C., Duran-Reynals F. THE MECHANISM OF ENHANCEMENT OF INFECTIONS BY TESTICLE EXTRACT. Science. 1930 Nov 14;72(1872):508–508. doi: 10.1126/science.72.1872.508. [DOI] [PubMed] [Google Scholar]
- Madinaveitia J. Studies on diffusing factors. III. Biochem J. 1939 Sep;33(9):1470–1477. doi: 10.1042/bj0331470. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Meyer K., Hobby G. L., Chaffee E., Dawson M. H. THE HYDROLYSIS OF HYALURONIC ACID BY BACTERIAL ENZYMES. J Exp Med. 1940 Jan 31;71(2):137–146. doi: 10.1084/jem.71.2.137. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pijoan M. THE ACTION OF TESTICLE, KIDNEY, AND SPLEEN EXTRACTS ON THE INFECTIVE POWER OF BACTERIA. J Exp Med. 1931 Jan 1;53(1):37–42. doi: 10.1084/jem.53.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]