Abstract
Suspensions of purified elementary bodies of vaccinia exhibit fluorescence in the presence of ultraviolet light. This fluorescent constituent can be separated by chromatographic methods provided the virus is first denatured by acid and heat. By means of the specific protein of d-amino acid oxidase it has been possible to identify the flavin constituent as flavin-adenine-dinucleotide and show that it can participate in the oxidative deamination of d-alanine. By means of microbiological assay the flavin component has been quantitatively measured and shown to compare favorably in concentration with that observed in animal cells and in some bacteria; its concentration in virus is lower than that observed in yeast. The demonstration that it exists as an integral portion of the virus is not conclusive. So far, however, it has been separated from the elementary bodies only by means which in themselves inactivate the virus.
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Selected References
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- Hoagland C. L., Lavin G. I., Smadel J. E., Rivers T. M. CONSTITUENTS OF ELEMENTARY BODIES OF VACCINIA : II. PROPERTIES OF NUCLEIC ACID OBTAINED FROM VACCINE VIRUS. J Exp Med. 1940 Jul 31;72(2):139–147. doi: 10.1084/jem.72.2.139. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hoagland C. L., Smadel J. E., Rivers T. M. CONSTITUENTS OF ELEMENTARY BODIES OF VACCINIA : I. CERTAIN BASIC ANALYSES AND OBSERVATIONS ON LIPID COMPONENTS OF THE VIRUS. J Exp Med. 1940 May 31;71(6):737–750. doi: 10.1084/jem.71.6.737. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hoagland C. L., Ward S. M., Smadel J. E., Rivers T. M. CONSTITUENTS OF ELEMENTARY BODIES OF VACCINIA : IV. DEMONSTRATION OF COPPER IN THE PURIFIED VIRUS. J Exp Med. 1941 Jun 30;74(1):69–80. doi: 10.1084/jem.74.1.69. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Krebs H. A. Metabolism of amino-acids: Deamination of amino-acids. Biochem J. 1935 Jul;29(7):1620–1644. doi: 10.1042/bj0291620. [DOI] [PMC free article] [PubMed] [Google Scholar]