Abstract
1. The agglutination titer for chicken red cells of freshly prepared or carefully stored suspensions of PR8 influenza virus, that is to say virus of maximum pathogenicity, was found to be proportional to the mouse lethal titer of the same preparations. 2. The agglutination titer of infected allantoic fluid procured in a standard way is relatively constant, regardless of the influenza strain used and its pathogenicity for mice. 3. Virus preparations inactivated by heat or storage may retain their agglutinating power. 4. Certain animal sera contain a partially heat-labile factor which, in low dilution, inhibits the agglutination of chicken red cells by influenza A and influenza B viruses. 5. The agglutination inhibition test, using ferret and human sera, gives qualitative data regarding influenza antibodies which are similar to the information obtained on the same sera by means of the virus neutralization test. 6. There is a definite relationship between the agglutination inhibition titer and the virus neutralization titer of a serum. On a logarithmic scale of both variables, this relationship is essentially linear within the range investigated. 7. The agglutination inhibition titer of immune ferret serum is inversely proportional to the amount of virus used in the test.
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Selected References
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- Francis T., Jr A NEW TYPE OF VIRUS FROM EPIDEMIC INFLUENZA. Science. 1940 Nov 1;92(2392):405–408. doi: 10.1126/science.92.2392.405. [DOI] [PubMed] [Google Scholar]
- Francis T., Shope R. E. NEUTRALIZATION TESTS WITH SERA OF CONVALESCENT OR IMMUNIZED ANIMALS AND THE VIRUSES OF SWINE AND HUMAN INFLUENZA. J Exp Med. 1936 Apr 30;63(5):645–653. doi: 10.1084/jem.63.5.645. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hirst G. K. THE AGGLUTINATION OF RED CELLS BY ALLANTOIC FLUID OF CHICK EMBRYOS INFECTED WITH INFLUENZA VIRUS. Science. 1941 Jul 4;94(2427):22–23. doi: 10.1126/science.94.2427.22. [DOI] [PubMed] [Google Scholar]
- Horsfall F. L., Hahn R. G., Rickard E. R. FOUR RECENT INFLUENZA EPIDEMICS: AN EXPERIMENTAL STUDY. J Clin Invest. 1940 Mar;19(2):379–392. doi: 10.1172/JCI101140. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Horsfall F. L., Lennette E. H., Rickard E. R. A COMPLEX VACCINE AGAINST INFLUENZA A VIRUS : QUANTITATIVE ANALYSIS OF THE ANTIBODY RESPONSE PRODUCED IN MAN. J Exp Med. 1941 Feb 28;73(3):335–355. doi: 10.1084/jem.73.3.335. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Horsfall F. L. NEUTRALIZATION OF EPIDEMIC INFLUENZA VIRUS : THE LINEAR RELATIONSHIP BETWEEN THE QUANTITY OF SERUM AND THE QUANTITY OF VIRUS NEUTRALIZED. J Exp Med. 1939 Jul 31;70(2):209–222. doi: 10.1084/jem.70.2.209. [DOI] [PMC free article] [PubMed] [Google Scholar]