Abstract
A considerable fraction of the influenza A virus contained in infected allantoic fluid of the developing chick is not sedimentable under conditions which remove virus activity almost completely from filtrates of emulsified mouse lung. The infectious unit from tissue suspensions is about 100 mµ in diameter and is of the same chemical composition as particles of the same size and abundance separated from normal tissues by an identical procedure. Evidence has been presented showing that the infectivity can be, and probably is, carried on such normal cell components as an adsorbate. Other non-infective particles such as erythrocytes may also become infectious units through adsorption of the virus. The virus occurs in allantoic fluid in two states of dispersion. A variable percentage is associated with particles considerably less than 100 mµ in diameter, probably more nearly 10 mµ, while the remainder is reversibly aggregated. Reversal to the more disperse state may be effected by dilution, sonic vibration, or moderate heat treatment.
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Selected References
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- Claude A. CHEMICAL COMPOSITION OF THE TUMOR-PRODUCING FRACTION OF CHICKEN TUMOR I. Science. 1939 Sep 1;90(2331):213–214. doi: 10.1126/science.90.2331.213. [DOI] [PubMed] [Google Scholar]
- Francis T., Jr TRANSMISSION OF INFLUENZA BY A FILTERABLE VIRUS. Science. 1934 Nov 16;80(2081):457–459. doi: 10.1126/science.80.2081.457-a. [DOI] [PubMed] [Google Scholar]
- Henle W., Chambers L. A., Groupé V. THE SEROLOGICAL SPECIFICITY OF PARTICULATE COMPONENTS DERIVED FROM VARIOUS NORMAL MAMMALIAN ORGANS. J Exp Med. 1941 Oct 31;74(5):495–510. doi: 10.1084/jem.74.5.495. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Henle W., Chambers L. A. SEROLOGICAL SPECIFICITY OF HEAVY PARTICLES DERIVED FROM NORMAL ORGANS. Science. 1940 Oct 4;92(2388):313–315. doi: 10.1126/science.92.2388.313. [DOI] [PubMed] [Google Scholar]
- Hirst G. K. THE AGGLUTINATION OF RED CELLS BY ALLANTOIC FLUID OF CHICK EMBRYOS INFECTED WITH INFLUENZA VIRUS. Science. 1941 Jul 4;94(2427):22–23. doi: 10.1126/science.94.2427.22. [DOI] [PubMed] [Google Scholar]
- Kabat E. A., Furth J. CHEMICAL AND IMMUNOLOGICAL STUDIES ON THE AGENT PRODUCING LEUKOSIS AND SARCOMA OF FOWLS. J Exp Med. 1940 Jan 1;71(1):55–70. doi: 10.1084/jem.71.1.55. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rawson A. J., Scherp H. W., Lindquist F. E. A Motor-Driven, Mechanically Supported Ultracentrifuge for the Separation of Biological Materials. J Bacteriol. 1940 Nov;40(5):657–663. doi: 10.1128/jb.40.5.657-663.1940. [DOI] [PMC free article] [PubMed] [Google Scholar]