Abstract
1. A method for the separation of chromatin threads from the resting nucleus of leukemic cells has been described. 2. The isolation of the chromatin strands was accomplished by purely mechanical means, and the separation, including extraction and purification in the centrifuge, required only about 1 hour. 3. Evidence is presented to show that the chromatin strands are morphologically related to the chromosomes, if not identical. 4. It seems probable that, by the present technique, chromatin strands can be obtained from different mammalian tissues for direct microscopical examination and biochemical analysis.
Full Text
The Full Text of this article is available as a PDF (877.1 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Crossmon G. THE ISOLATION OF MUSCLE NUCLEI. Science. 1937 Mar 5;85(2201):250–250. doi: 10.1126/science.85.2201.250. [DOI] [PubMed] [Google Scholar]
- Potter J. S., Richter M. N. Studies on Mouse Leukemia: VI. The Predominating Cell Type in Line I. Proc Natl Acad Sci U S A. 1932 Apr;18(4):298–303. doi: 10.1073/pnas.18.4.298. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Richter M. N., Macdowell E. C. STUDIES ON LEUKEMIA IN MICE : I. THE EXPERIMENTAL TRANSMISSION OF LEUKEMIA. J Exp Med. 1930 Mar 31;51(4):659–673. doi: 10.1084/jem.51.4.659. [DOI] [PMC free article] [PubMed] [Google Scholar]