Abstract
Comparative immunological measurements have been carried out on crystalline horse serum albumin in the native state and after regeneration from 8 M urea solutions. The mean antigenic activity of the regenerated protein has been found to be less than 10 per cent of that of the native, whereas both antigens proved to be immunologically equivalent. The problem of the relation between protein denaturation and immunological activity has been considered and discussed on the basis of known physical and chemical differences between native and denatured protein.
Full Text
The Full Text of this article is available as a PDF (403.5 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Heidelberger M., Kendall F. E. THE PRECIPITIN REACTION BETWEEN TYPE III PNEUMOCOCCUS POLYSACCHARIDE AND HOMOLOGOUS ANTIBODY : II. CONDITIONS FOR QUANTITATIVE PRECIPITATION OF ANTIBODY IN HORSE SERA. J Exp Med. 1935 Mar 31;61(4):559–562. doi: 10.1084/jem.61.4.559. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hewitt L. F. The antigenic behaviour of serum proteins with special reference to crystalbumin and seroglycoid. Biochem J. 1937 Jul;31(7):1047–1052. doi: 10.1042/bj0311047. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kekwick R. A. Observations on the crystallizable albumin fraction of horse serum. Biochem J. 1938 Mar;32(3):552–562. doi: 10.1042/bj0320552. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rothen A., Landsteiner K. SEROLOGICAL REACTIONS OF PROTEIN FILMS AND DENATURED PROTEINS. J Exp Med. 1942 Nov 1;76(5):437–450. doi: 10.1084/jem.76.5.437. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smetana H., Shemin D. STUDIES ON PHOTO-OXIDATION OF ANTIGEN AND ANTIBODIES. J Exp Med. 1941 Jan 31;73(2):223–242. doi: 10.1084/jem.73.2.223. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Taylor A. R., Beard J. W. AN INEXPENSIVE APPARATUS FOR DRYING FROM THE FROZEN STATE. Science. 1940 Dec 27;92(2400):611–612. doi: 10.1126/science.92.2400.611. [DOI] [PubMed] [Google Scholar]
- Tiselius A., Kabat E. A. AN ELECTROPHORETIC STUDY OF IMMUNE SERA AND PURIFIED ANTIBODY PREPARATIONS. J Exp Med. 1939 Jan 1;69(1):119–131. doi: 10.1084/jem.69.1.119. [DOI] [PMC free article] [PubMed] [Google Scholar]