Abstract
1. Strains of staphylococci produce diazotizable materials which can be converted to a dye, and the intensity of the color reaction can be quantitated in the same manner as p-aminobenzoic acid. The sulfonamide-resistant strains which we have studied produce more diazotizable substance than non-resistant strains. 2. The development of a color by the diazotizable substance can be inhibited by exposing the substance to a soil bacillus (Mirick) specifically adapted to oxidize PABA. 3. The diazotizable substance produced by staphylococci inhibits the anti-staphylococcic action of sodium sulfathiazole to approximately the same degree as equivalent amounts of pure p-aminobenzoic acid. 4. Two microbiological methods for assaying p-aminobenzoic acid were employed for quantitating the amount of this material produced by staphylococci. In general, the sulfonamide-resistant strains produced more p-aminobenzoic acid than the sulfonamide-sensitive strains. The inconstant results obtained with these biological assays are discussed.
Full Text
The Full Text of this article is available as a PDF (485.1 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Landy M., Larkum N. W., Oswald E. J., Streightoff F. INCREASED SYNTHESIS OF p-AMINOBENZOIC ACID ASSOCIATED WITH THE DEVELOPMENT OF SULFONAMIDE RESISTANCE IN STAPHYLOCOCCUS AUREUS. Science. 1943 Mar 19;97(2516):265–267. doi: 10.1126/science.97.2516.265. [DOI] [PubMed] [Google Scholar]
- Macleod C. M. THE INHIBITION OF THE BACTERIOSTATIC ACTION OF SULFONAMIDE DRUGS BY SUBSTANCES OF ANIMAL AND BACTERIAL ORIGIN. J Exp Med. 1940 Aug 31;72(3):217–232. doi: 10.1084/jem.72.3.217. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mirick G. S. THE OXIDATION OF p-AMINOBENZOIC ACID AND ANTHRANILIC ACID BY SPECIFICALLY ADAPTED ENZYMES OF A SOIL BACILLUS. J Exp Med. 1943 Oct 1;78(4):255–272. doi: 10.1084/jem.78.4.255. [DOI] [PMC free article] [PubMed] [Google Scholar]