Abstract
Manometric determinations of cytochrome oxidase activity were carried out on grey matter from the thalamus and anterior horn of cats and monkeys under various experimental conditions. The thalamus of the cat was studied following the degeneration of virtually all the thalamic neurons secondary to decortication. In comparing the deneuronated thalamus with the normal one, it was found that approximately 34 per cent of the cytochrome oxidase activity was contributed by the neurons and the balance by neuroglia and mesodermal tissues which on the operated side remained comparable to that of the normal side. Total activity of the normal thalamus averaged 5.52 units per mg. of dry weight where I unit is defined as the amount of cytochrome oxidase required to produce a net oxygen consumption of 10 c.mm. per hour under the specified conditions of the experiment. The grey matter of the anterior horns of the spinal cord was isolated by a special technique and its cytochrome oxidase activity was compared with anterior horns in which motoneurons had been stimulated to regenerative activity by section of peripheral nerves. Each animal was studied in relation to an anterior horn which was normal and one in which only the functional state of the motoneurons had been changed. Average normal levels of 2.23 units were found for cat anterior horn and 0.69 units for the monkey. Reductions of cytochrome oxidase activity in the range of 22 to 23 per cent were observed for both cat and monkey following nerve section. In the latter the time sequence was carefully studied in relation to the cytological cycle known as chromatolysis and a virus refractory state previously described by us. It was found that maximal reduction of cytochrome oxidase activity coincided with maximal refractoriness of the cells to poliomyelitis virus (30 to 70 days following nerve section). Neither of these states could be correlated in time with maximal chromatolysis (10 to 15 days).
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Selected References
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- Bodian D., Mellors R. C. THE REGENERATIVE CYCLE OF MOTONEURONS, WITH SPECIAL REFERENCE TO PHOSPHATASE ACTIVITY. J Exp Med. 1945 May 1;81(5):469–488. doi: 10.1084/jem.81.5.469. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Foster C., Jones J. H., Henle W., Dorfman F. THE EFFECT OF VITAMIN B(1) DEFICIENCY AND OF RESTRICTED FOOD INTAKE ON THE RESPONSE OF MICE TO THE LANSING STRAIN OF POLIOMYELITIS VIRUS. J Exp Med. 1944 Feb 1;79(2):221–234. doi: 10.1084/jem.79.2.221. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Friedewald W. F. CELL STATE AS AFFECTING SUSCEPTIBILITY TO A VIRUS : ENHANCED EFFECTIVENESS OF THE RABBIT PAPILLOMA VIRUS ON HYPERPLASTIC EPIDERMIS. J Exp Med. 1942 Jan 31;75(2):197–220. doi: 10.1084/jem.75.2.197. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Parker R. F., Thompson R. L. THE EFFECT OF EXTERNAL TEMPERATURE ON THE COURSE OF INFECTIOUS MYXOMATOSIS OF RABBITS. J Exp Med. 1942 Jun 1;75(6):567–573. doi: 10.1084/jem.75.6.567. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Racker E., Kabat H. THE METABOLISM OF THE CENTRAL NERVOUS SYSTEM IN EXPERIMENTAL POLIOMYELITIS. J Exp Med. 1942 Dec 1;76(6):579–585. doi: 10.1084/jem.76.6.579. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sabin A. B., Duffy C. E. NUTRITION AS A FACTOR IN THE DEVELOPMENT OF CONSTITUTIONAL BARRIERS TO INVOLVEMENT OF THE NERVOUS SYSTEM BY CERTAIN VIRUSES. Science. 1940 Jun 7;91(2371):552–554. doi: 10.1126/science.91.2371.552. [DOI] [PubMed] [Google Scholar]