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The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1945 Aug 1;82(2):119–132. doi: 10.1084/jem.82.2.119

BACTERIOSTATIC EFFECT OF HUMAN SERA ON GROUP A STREPTOCOCCI

III. INTERFERENCE WITH BACTERIOSTATIC ACTIVITY BY BLOCKAGE OF THE LEUKOCYTES

Sidney Rothbard 1
PMCID: PMC2135544  PMID: 19871487

Abstract

1. Type-specific M extracts and group-specific C carbohydrate of group A streptococci inhibited bacteriostasis of these microorganisms in the presence of normal whole blood and of sera from patients convalescent from streptococcal infections. The inhibition was not specific with respect to streptococcal types and depended merely on the formation of precipitates in the system. The extracts had no antagonistic action in themselves. 2. Preformed precipitates derived from the interaction of an antigen and its homologous antibody or from finely divided coagulated particles of human plasma or ascitic fluid also interfered with the bacteriostasis. The supernatant fluid in which one of these precipitates was formed did not inhibit bacteriostatic activity; therefore, it seems that other possible products of the antigen-antibody reaction were not inhibitory. The relative size of the precipitate particles was a conditioning factor since small particles of one precipitate inhibited bacteriostasis, but large ones of the same precipitate failed to do so. 3. Stained films of blood cells treated with antigen-antibody mixtures which formed a precipitate revealed large cytoplasmic vacuoles containing precipitates in the polymorphonuclear leukocytes and monocytes; such engorged cells subsequently failed to phagocytize streptococci in homologous serum. Blood cells treated in the same manner, except that the antigen-antibody mixtures formed no precipitate, contained no vacuoles, and these cells were able to phagocytize the streptococci. 4. Leukocytes studied in the living state in the presence of colored precipitate and streptococci sensitized by convalescent human serum showed unselective phagocytosis of both precipitate and bacteria. The capacity of these leukocytes to ingest material however was limited. 5. As a result of non-selective saturation of their phagocytic capacity in the bacteriostatic systems containing both streptococci and precipitate, the limited number of leukocytes phagocytized only a fraction of the streptococci; consequently the remainder were able to multiply.

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Selected References

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  1. Heidelberger M., Kendall F. E. SPECIAL ARTICLES. Science. 1930 Sep 5;72(1862):252–253. doi: 10.1126/science.72.1862.252. [DOI] [PubMed] [Google Scholar]
  2. Lancefield R. C. THE ANTIGENIC COMPLEX OF STREPTOCOCCUS HAEMOLYTICUS : I. DEMONSTRATION OF A TYPE-SPECIFIC SUBSTANCE IN EXTRACTS OF STREPTOCOCCUS HAEMOLYTICUS. J Exp Med. 1928 Jan 1;47(1):91–103. doi: 10.1084/jem.47.1.91. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Rothbard S. BACTERIOSTATIC EFFECT OF HUMAN SERA ON GROUP A STREPTOCOCCI : I. TYPE-SPECIFIC ANTIBODIES IN SERA OF PATIENTS CONVALESCING FROM GROUP A STREPTOCOCCAL PHARYNGITIS. J Exp Med. 1945 Aug 1;82(2):93–106. doi: 10.1084/jem.82.2.93. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Sabin F. R. CELLULAR REACTIONS TO A DYE-PROTEIN WITH A CONCEPT OF THE MECHANISM OF ANTIBODY FORMATION. J Exp Med. 1939 Jun 30;70(1):67–82. doi: 10.1084/jem.70.1.67. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Sia R. H. STUDIES ON PNEUMOCOCCUS GROWTH INHIBITION : VI. THE SPECIFIC EFFECT OF PNEUMOCOCCUS SOLUBLE SUBSTANCE ON THE GROWTH OF PNEUMOCOCCI IN NORMAL SERUM-LEUCOCYTE MIXTURES. J Exp Med. 1926 Apr 30;43(5):633–645. doi: 10.1084/jem.43.5.633. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Victor J., Van Buren J. R., Smith H. P. STUDIES ON VITAL STAINING : IV. INDIA INK AND BRILLIANT VITAL RED. IMPORTANCE OF CONSIDERING LIVER EXCRETION IN THE STUDY OF "BLOCKADE OF THE RETICULO-ENDOTHELIAL SYSTEM". J Exp Med. 1930 Mar 31;51(4):531–548. doi: 10.1084/jem.51.4.531. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ward H. K., Enders J. F. AN ANALYSIS OF THE OPSONIC AND TROPIC ACTION OF NORMAL AND IMMUNE SERA BASED ON EXPERIMENTS WITH THE PNEUMOCOCCUS. J Exp Med. 1933 Mar 31;57(4):527–547. doi: 10.1084/jem.57.4.527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ward H. K. OBSERVATIONS ON THE PHAGOCYTOSIS OF THE PNEUMOCOCCUS BY HUMAN WHOLE BLOOD : I. THE NORMAL PHAGOCYTIC TITRE, AND THE ANTI-PHAGOCYTIC EFFECT OF THE SPECIFIC SOLUBLE SUBSTANCE. J Exp Med. 1930 Apr 30;51(5):675–684. doi: 10.1084/jem.51.5.675. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ward H. K. OBSERVATIONS ON THE PHAGOCYTOSIS OF THE PNEUMOCOCCUS BY HUMAN WHOLE BLOOD : II. THE NEUTRALIZATION OF THE ANTI-PHAGOCYTIC ACTION OF THE SPECIFIC SOLUBLE SUBSTANCE BY ANTISERUM. J Exp Med. 1930 Apr 30;51(5):685–702. doi: 10.1084/jem.51.5.685. [DOI] [PMC free article] [PubMed] [Google Scholar]

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