Abstract
Following the injection of dysentery antigen in saline or in saline-in-paraffin-oil emulsion into the pad of the rabbit's hind foot, considerable quantities of antibody were recovered from the popliteal lymph node, while the tissue at the site of injection, containing many granulocytes and numerous macrophages, revealed only insignificant quantities of antibody. Following the injection of various dysentery and typhoid antigen combinations into the abdominal cavity, no antibody was found in the isolated granulocytes and macrophages of the peritoneal exudate, while the supernatant fluid revealed titers that roughly paralleled those of the blood serum. Similar results were obtained when animals were injected first with antibody intravenously, and subsequently with an unspecific irritant intraperitoneally. The presence of antibody in the supernatant fluid was, therefore, interpreted as being due to secondary concentration (fixation) in an inflamed area. These findings together with the previously described observations on the lymphocyte seem to show that the macrophage does not synthesize agglutinins against dysentery or typhoid bacilli.
Full Text
The Full Text of this article is available as a PDF (509.0 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ehrich W. E., Halbert S. P., Mertens E., Mudd S. MECHANISM OF THE AUGMENTING ACTION OF MINERAL OIL ON ANTIBODY PRODUCTION : TISSUE REACTIONS AND ANTIBODY RESPONSE TO DYSENTERY VACCINE IN SALINE, AND IN SALINE-LANOLIN-MINERAL OIL EMULSION. J Exp Med. 1945 Oct 31;82(5):343–360. doi: 10.1084/jem.82.5.343. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ehrich W. E., Harris T. N. THE FORMATION OF ANTIBODIES IN THE POPLITEAL LYMPH NODE IN RABBITS. J Exp Med. 1942 Oct 1;76(4):335–348. doi: 10.1084/jem.76.4.335. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ehrich W. E., Harris T. N. THE SITE OF ANTIBODY FORMATION. Science. 1945 Jan 12;101(2611):28–31. doi: 10.1126/science.101.2611.28. [DOI] [PubMed] [Google Scholar]
- Harris T. N., Grimm E., Mertens E., Ehrich W. E. THE ROLE OF THE LYMPHOCYTE IN ANTIBODY FORMATION. J Exp Med. 1945 Jan 1;81(1):73–83. doi: 10.1084/jem.81.1.73. [DOI] [PMC free article] [PubMed] [Google Scholar]