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. 1947 Mar 31;85(4):381–394. doi: 10.1084/jem.85.4.381

A MANOMETRIC METHOD FOR MEASURING THE ACTIVITY OF THE CL. WELCHII LECITHINASE AND A DESCRIPTION OF CERTAIN PROPERTIES OF THIS ENZYME

Paul C Zamecnik 1, Lydia E Brewster 1, Fritz Lipmann 1
PMCID: PMC2135613  PMID: 19871623

Abstract

A manometric method has been described for measurement of the activity of Cl. welchii lecithinase. The method depends on displacement of carbon dioxide from a bicarbonate-carbon dioxide-buffered medium by the phosphorylcholine liberated during the enzymatic hydrolysis.

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Selected References

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  1. Macfarlane M. G., Knight B. C. The biochemistry of bacterial toxins: The lecithinase activity of Cl. welchii toxins. Biochem J. 1941 Sep;35(8-9):884–902. doi: 10.1042/bj0350884. [DOI] [PMC free article] [PubMed] [Google Scholar]
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