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. 1946 Oct 31;84(5):449–471. doi: 10.1084/jem.84.5.449

THE PROPERTIES OF T ANTIGENS EXTRACTED FROM GROUP A HEMOLYTIC STREPTOCOCCI

Rebecca C Lancefield 1, Vincent P Dole 1
PMCID: PMC2135665  PMID: 19871581

Abstract

1. T antigens of group A hemolytic streptococci have been obtained in soluble form by digestion of the bacterial cells with pepsin or trypsin. Large quantities of this antigen were readily extracted from type 1 strains, whereas only small amounts could be obtained from strains of other types. 2. The T antigen, prepared in this way from a type 1 strain, was partially purified by chemical precipitation and further enzymatic digestion. An active fraction, apparently protein in nature, was separated electrophoretically at pH 7.00. The separated material, pooled and analyzed at the same pH, gave only a single peak. The isoelectric point of this substance was about pH 4.50. An elementary analysis was obtained. Although the T antigen was resistant to digestion with proteolytic enzymes and ribonuclease, it was readily inactivated by heat, especially in acid media and in strong salt solutions. The serological activity of this purified T substance was lost after exposure to ultraviolet radiation. 3. Analysis by means of the ultracentrifuge showed that the material was polydisperse and therefore probably impure. 4. The soluble form of the T substance was active in the precipitin reaction, in the fixation of complement, in inhibition of T agglutination, and as an antigen when injected into rabbits. The antibodies produced did not protect mice against infection with virulent strains of hemolytic streptococci containing the same T antigen. 5. The immunological specificity of T antigen in soluble form is the same as that of the T antigen in the intact streptococcus from which it was derived

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Selected References

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  1. Havens W. P., Watson D. W., Green R. H., Lavin G. I., Smadel J. E. COMPLEMENT FIXATION WITH THE NEUROTROPIC VIRUSES. J Exp Med. 1943 Feb 1;77(2):139–153. doi: 10.1084/jem.77.2.139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Hirst G. K., Lancefield R. C. ANTIGENIC PROPERTIES OF THE TYPE-SPECIFIC SUBSTANCE DERIVED FROM GROUP A HEMOLYTIC STREPTOCOCCI. J Exp Med. 1939 Feb 28;69(3):425–445. doi: 10.1084/jem.69.3.425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Krumwiede E. A Comparison of the Value of the Agglutination and Precipitin Reactions in the Serological Typing of Group A Streptococci. J Bacteriol. 1943 Aug;46(2):117–131. doi: 10.1128/jb.46.2.117-131.1943. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kuttner A. G., Lenert T. F. THE OCCURRENCE OF BACTERIOSTATIC PROPERTIES IN THE BLOOD OF PATIENTS AFTER RECOVERY FROM STREPTOCOCCAL PHARYNGITIS. J Clin Invest. 1944 Mar;23(2):151–161. doi: 10.1172/JCI101478. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lancefield R. C. A SEROLOGICAL DIFFERENTIATION OF HUMAN AND OTHER GROUPS OF HEMOLYTIC STREPTOCOCCI. J Exp Med. 1933 Mar 31;57(4):571–595. doi: 10.1084/jem.57.4.571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lancefield R. C., Hare R. THE SEROLOGICAL DIFFERENTIATION OF PATHOGENIC AND NON-PATHOGENIC STRAINS OF HEMOLYTIC STREPTOCOCCI FROM PARTURIENT WOMEN. J Exp Med. 1935 Feb 28;61(3):335–349. doi: 10.1084/jem.61.3.335. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lancefield R. C. STUDIES ON THE ANTIGENIC COMPOSITION OF GROUP A HEMOLYTIC STREPTOCOCCI : I. EFFECTS OF PROTEOLYTIC ENZYMES ON STREPTOCOCCAL CELLS. J Exp Med. 1943 Dec 1;78(6):465–476. doi: 10.1084/jem.78.6.465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lancefield R. C., Stewart W. A. STUDIES ON THE ANTIGENIC COMPOSITION OF GROUP A HEMOLYTIC STREPTOCOCCI : II. THE OCCURRENCE OF STRAINS IN A GIVEN TYPE CONTAINING M BUT NO T ANTIGEN. J Exp Med. 1944 Jan 1;79(1):79–88. doi: 10.1084/jem.79.1.79. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lancefield R. C. THE ANTIGENIC COMPLEX OF STREPTOCOCCUS HAEMOLYTICUS : II. CHEMICAL AND IMMUNOLOGICAL PROPERTIES OF THE PROTEIN FRACTIONS. J Exp Med. 1928 Feb 29;47(3):469–480. doi: 10.1084/jem.47.3.469. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lancefield R. C. THE SIGNIFICANCE OF M AND T ANTIGENS IN THE CROSS REACTIONS BETWEEN CERTAIN TYPES OF GROUP A HEMOLYTIC STREPTOCOCCI. J Exp Med. 1940 Mar 31;71(4):539–550. doi: 10.1084/jem.71.4.539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lancefield R. C. TYPE-SPECIFIC ANTIGENS, M AND T, OF MATT AND GLOSSY VARIANTS OF GROUP A HEMOLYTIC STREPTOCOCCI. J Exp Med. 1940 Mar 31;71(4):521–537. doi: 10.1084/jem.71.4.521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lancefield R. C., Todd E. W. ANTIGENIC DIFFERENCES BETWEEN MATT HEMOLYTIC STREPTOCOCCI AND THEIR GLOSSY VARIANTS. J Exp Med. 1928 Nov 30;48(6):769–790. doi: 10.1084/jem.48.6.769. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rothbard S. BACTERIOSTATIC EFFECT OF HUMAN SERA ON GROUP A STREPTOCOCCI : I. TYPE-SPECIFIC ANTIBODIES IN SERA OF PATIENTS CONVALESCING FROM GROUP A STREPTOCOCCAL PHARYNGITIS. J Exp Med. 1945 Aug 1;82(2):93–106. doi: 10.1084/jem.82.2.93. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Stewart W. A., Lancefield R. C., Wilson A. T., Swift H. F. STUDIES ON THE ANTIGENIC COMPOSITION OF GROUP A HEMOLYTIC STREPTOCOCCI : IV. RELATED T BUT DISTINCT M ANTIGENS IN TYPES 15, 17, 19, 23, 30, AND IN TYPES 4, 24, 26, 28, 29, 46. IDENTIFICATION BY SLIDE AGGLUTINATION. J Exp Med. 1944 Jan 1;79(1):99–114. doi: 10.1084/jem.79.1.99. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Swift H. F., Wilson A. T., Lancefield R. C. TYPING GROUP A HEMOLYTIC STREPTOCOCCI BY M PRECIPITIN REACTIONS IN CAPILLARY PIPETTES. J Exp Med. 1943 Aug 1;78(2):127–133. doi: 10.1084/jem.78.2.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Todd E. W., Lancefield R. C. VARIANTS OF HEMOLYTIC STREPTOCOCCI; THEIR RELATION TO TYPE-SPECIFIC SUBSTANCE, VIRULENCE, AND TOXIN. J Exp Med. 1928 Nov 30;48(6):751–767. doi: 10.1084/jem.48.6.751. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Watson R. F., Lancefield R. C. STUDIES ON THE ANTIGENIC COMPOSITION OF GROUP A HEMOLYTIC STREPTOCOCCI : III. TYPES WITH SEROLOGICALLY IDENTICAL M BUT DISTINCT T ANTIGENS: TYPES 10 AND 12. J Exp Med. 1944 Jan 1;79(1):89–98. doi: 10.1084/jem.79.1.89. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Watson R. F., Rothbard S., Swift H. F., Technical Assistance of Gilbert de Mello TYPE-SPECIFIC PROTECTION AND IMMUNITY FOLLOWING INTRANASAL INOCULATION OF MONKEYS WITH GROUP A HEMOLYTIC STREPTOCOCCI. J Exp Med. 1946 Jul 31;84(2):127–142. [PMC free article] [PubMed] [Google Scholar]

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