Abstract
A technique has been devised for obtaining free or uncombined pneumonia virus of mice (PVM). Free PVM, liberated from infected mouse lungs by means of this technique, is infectious and causes hemagglutination directly. The results of quantitative studies carried out in the high speed angle centrifuge indicate that the free virus is relatively small, with dimensions of the order of 40 millimicrons. When it is in combination with lung tissue particles, PVM appears to be a relatively large virus with minimal dimensions of the order of 140 millimicrons. Non-infectious virus particles, released from combination with lung tissue particles by heating, are similar to the infectious free virus in size.
Full Text
The Full Text of this article is available as a PDF (854.1 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bauer J. H., Pickels E. G. A HIGH SPEED VACUUM CENTRIFUGE SUITABLE FOR THE STUDY OF FILTERABLE VIRUSES. J Exp Med. 1936 Sep 30;64(4):503–528. doi: 10.1084/jem.64.4.503. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Curnen E. C., Horsfall F. L. STUDIES ON PNEUMONIA VIRUS OF MICE (PVM) : III. HEMAGGLUTINATION BY THE VIRUS; THE OCCURRENCE OF COMBINATION BETWEEN THE VIRUS AND A TISSUE SUBSTANCE. J Exp Med. 1946 Jan 31;83(2):105–132. [PMC free article] [PubMed] [Google Scholar]
- Friedewald W. F., Pickels E. G. CENTRIFUGATION AND ULTRAFILTRATION STUDIES ON ALLANTOIC FLUID PREPARATIONS OF INFLUENZA VIRUS. J Exp Med. 1944 Mar 1;79(3):301–317. doi: 10.1084/jem.79.3.301. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Horsfall F. L., Curnen E. C. STUDIES ON PNEUMONIA VIRUS OF MICE (PVM) : II. IMMUNOLOGICAL EVIDENCE OF LATENT INFECTION WITH THE VIRUS IN NUMEROUS MAMMALIAN SPECIES. J Exp Med. 1946 Jan 1;83(1):43–64. [PMC free article] [PubMed] [Google Scholar]
- Horsfall F. L., Hahn R. G. A LATENT VIRUS IN NORMAL MICE CAPABLE OF PRODUCING PNEUMONIA IN ITS NATURAL HOST. J Exp Med. 1940 Feb 29;71(3):391–408. doi: 10.1084/jem.71.3.391. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hughes T. P., Pickels E. G., Horsfall F. L. A METHOD FOR DETERMINING THE DIFFERENTIAL SEDIMENTATION OF PROTEINS IN THE HIGH SPEED CONCENTRATION CENTRIFUGE. J Exp Med. 1938 May 31;67(6):941–952. doi: 10.1084/jem.67.6.941. [DOI] [PMC free article] [PubMed] [Google Scholar]