Abstract
The spreading response induced by intradermal administration of hyaluronidase is influenced not only by enzyme concentration, but also by the volume and pressure of the injection. These pressure-volume factors influence the rate of spreading and determine the final area of spreading. When a constant volume is injected, the rate of spreading to the area determined by the volume of injection is a function of the enzyme concentration. These and other findings have led to the conclusion that hyaluronidase is an effective spreading agent only when the slow diffusion of enzyme in skin is accelerated by a localized increase of interstitial pressure and volume. These considerations of hyaluronidase action in skin have been utilized to explain the shape of the dosage-response curve of hyaluronidase spreading activity, and the differences in the spreading reactions between crude snake venoms and purified hyaluronidase. The significance of the findings as related to the rôle of hyaluronidase in bacterial invasiveness, and in the assay of S. F. are briefly discussed.
Full Text
The Full Text of this article is available as a PDF (1.0 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bacharach A. L., Chance M. R., Middleton T. R. The biological assay of testicular diffusing factor. Biochem J. 1940 Nov;34(10-11):1464–1471. doi: 10.1042/bj0341464. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Duran-Reynals F. TISSUE PERMEABILITY AND THE SPREADING FACTORS IN INFECTION : A Contribution to the Host:Parasite Problem. Bacteriol Rev. 1942 Dec;6(4):197–252. doi: 10.1128/br.6.4.197-252.1942. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Evans M. G., Madinaveitia J. Studies on diffusing factors: The mode of action of diffusing factors. Biochem J. 1940 Apr;34(4):613–620. doi: 10.1042/bj0340613. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hoffman D. C., Duran-Reynals F. THE INFLUENCE OF TESTICLE EXTRACT ON THE INTRADERMAL SPREAD OF INJECTED FLUIDS AND PARTICLES. J Exp Med. 1931 Feb 28;53(3):387–398. doi: 10.1084/jem.53.3.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Humphrey J. H. Studies on diffusing factors: 3. A new biological assay of diffusing factor in guinea-pigs. Biochem J. 1943 Jul;37(2):177–181. doi: 10.1042/bj0370177. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Madinaveitia J. Diffusing factors: Concentration of the mucinase from testicular extracts and from Crotalus atrox venom. Biochem J. 1941 Apr;35(4):447–452. doi: 10.1042/bj0350447. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Madinaveitia J. Studies on diffusing factors. Active preparations from mammalian testicle and their biological assay. Biochem J. 1938 Oct;32(10):1806–1813. doi: 10.1042/bj0321806. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Madinaveitia J. Studies on diffusing factors. III. Biochem J. 1939 Sep;33(9):1470–1477. doi: 10.1042/bj0331470. [DOI] [PMC free article] [PubMed] [Google Scholar]