Abstract
A procedure has been detailed for the preparation of sterile non-pyrogenic solutions of oxyhemoglobin which have the approximate protein content and electrolyte composition of plasma. Large volumes of solution can be rapidly prepared, with 95 to 98 per cent of the hemoglobin in the active form capable of combining with oxygen. The solutions contain no particulate matter; 95 per cent of total blood lipids are removed. Solutions stored at 4°C. showed no conversion of hemoglobin to methemoglobin over a period of 2½ months; over a 6 month period a small and variable amount of methemoglobin may be formed.
Full Text
The Full Text of this article is available as a PDF (525.4 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Farr L. E., Hiller A., Van Slyke D. D., Technical Assistance of John Plazin PREPARATION OF DRIED HEMOGLOBIN WITHOUT LOSS OF ACTIVITY. J Exp Med. 1947 Nov 30;86(6):465–475. doi: 10.1084/jem.86.6.465. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gilligan D. R., Altschule M. D., Katersky E. M. STUDIES OF HEMOGLOBINEMIA AND HEMOGLOBINURIA PRODUCED IN MAN BY INTRAVENOUS INJECTION OF HEMOGLOBIN SOLUTIONS. J Clin Invest. 1941 Mar;20(2):177–187. doi: 10.1172/JCI101210. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jorpes E. The protein component of the erythrocyte membrane or stroma. Biochem J. 1932;26(5):1488–1503. doi: 10.1042/bj0261488. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Newman W. V., Whipple G. H. IV. HEMOGLOBIN INJECTIONS AND CONSERVATION OF PIGMENT BY KIDNEY, LIVER AND SPLEEN : THE INFLUENCE OF DIET AND BLEEDING. J Exp Med. 1932 Mar 31;55(4):637–652. doi: 10.1084/jem.55.4.637. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robscheit-Robbins F. S., Miller L. L., Alling E. L., Whipple G. H. PLASMA SUBSTITUTES : HUMAN AND ANIMAL GLOBIN RELATED TO THE PRODUCTION OF HEMOGLOBIN AND PLASMA PROTEIN DOG HEMOGLOBIN UTILIZATION IMPROVED BY METHIONINE BUT NOT BY ISOLEUCINE. J Exp Med. 1946 Apr 30;83(5):355–371. [PMC free article] [PubMed] [Google Scholar]