Abstract
1. It is likely that certain tailed and filamentous particles seen on electron microscope examination of partially purified saline suspensions of Newcastle virus are the individual virus particles because: (a) They have a highly characteristic shape not seen in other virus preparations. (b) They are present whenever the virus is present in high concentration. (c) Their size agrees with the size of the virus as calculated from light scattering and centrifuge data. (d) They are agglutinated by specific antisera. (e) Infection may be produced in the embryo by relatively few of these particles. 2. It is possible that these filamentous forms have been derived from spherical forms without loss of activity because: (a) Such filamentous forms are not found in the original allantoic fluid when this contains a comparable amount of virus. (b) Filamentous forms appeared in the original allantoic fluid when it was dialyzed against saline solution. (c) Filamentous forms were produced at certain hydrogen ion concentrations but not at others, in solutions maintaining the same infectivity for the embryo. (d) Spherical forms were obtained by suspending the partially purified virus in water instead of saline. In this the virus remained moderately stable. (e) These round forms could apparently be converted into tailed and filamentous forms by the addition of saline, again without loss of activity. (f) This "conversion" could be inhibited by partial inactivation of the water suspension of virus.
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Selected References
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- BANG F. B. Studies on Newcastle disease virus; an evaluation of the method of titration. J Exp Med. 1948 Aug;88(2):233–240. doi: 10.1084/jem.88.2.233. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BANG F. B. Studies on Newcastle disease virus; behavior of the virus in the embryo. J Exp Med. 1948 Aug;88(2):241–249. doi: 10.1084/jem.88.2.241. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lauffer M. A., Stanley W. M. BIOPHYSICAL PROPERTIES OF PREPARATIONS OF PR8 INFLUENZA VIRUS. J Exp Med. 1944 Dec 1;80(6):531–548. doi: 10.1084/jem.80.6.531. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Oster G. Molecular Weights and Other Properties of Viruses as Determined by Light Absorption. Science. 1946 Mar 8;103(2671):306–308. doi: 10.1126/science.103.2671.306. [DOI] [PubMed] [Google Scholar]
- Parker R. F., Bronson L. H., Green R. H. FURTHER STUDIES OF THE INFECTIOUS UNIT OF VACCINIA. J Exp Med. 1941 Aug 31;74(3):263–281. doi: 10.1084/jem.74.3.263. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Proceedings of the Biochemical Society. Biochem J. 1947;41(2):xxv–xxxiv. [PMC free article] [PubMed] [Google Scholar]
- Wyckoff R. W. Some Recent Developments in the Field of Electron Microscopy. Science. 1946 Jul 12;104(2689):21–26. doi: 10.1126/science.104.2689.21. [DOI] [PubMed] [Google Scholar]