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. 1986 Nov;168(2):1005–1009. doi: 10.1128/jb.168.2.1005-1009.1986

Use of the Bacillus subtilis subtilisin signal peptide for efficient secretion of TEM beta-lactamase during growth.

S L Wong, F Kawamura, R H Doi
PMCID: PMC213584  PMID: 3096954

Abstract

We report the development of an efficient Bacillus subtilis secretory system, with the secreted product stably maintained in the medium for 100 h. The system is based on characterization of the subtilisin signal peptidase cleavage site and promoters, catabolite repression of sporulation, presence of a vegetative secreting mechanism, and availability of a protease-deficient strain.

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Selected References

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