Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1986 Nov;168(2):1014–1018. doi: 10.1128/jb.168.2.1014-1018.1986

Expression of the bacteriophage T4 denV structural gene in Escherichia coli.

A Recinos 3rd, M L Augustine, K M Higgins, R S Lloyd
PMCID: PMC213586  PMID: 3536845

Abstract

The expression of the T4 denV gene, which previously had been cloned in plasmid constructs downstream of the bacteriophage lambda hybrid promoter-operator oLpR, was analyzed under a variety of growth parameters. Expression of the denV gene product, endonuclease V, was confirmed in DNA repair-deficient Escherichia coli (uvrA recA) by Western blot analyses and by enhancements of resistance to UV irradiation.

Full text

PDF
1014

Images in this article

1016Image
on p.1016
1018Image
on p.1018

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev. 1972 Dec;36(4):525–557. doi: 10.1128/br.36.4.525-557.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Burnette W. N. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem. 1981 Apr;112(2):195–203. doi: 10.1016/0003-2697(81)90281-5. [DOI] [PubMed] [Google Scholar]
  3. Chenevert J. M., Naumovski L., Schultz R. A., Friedberg E. C. Partial complementation of the UV sensitivity of E. coli and yeast excision repair mutants by the cloned denV gene of bacteriophage T4. Mol Gen Genet. 1986 Apr;203(1):163–171. doi: 10.1007/BF00330398. [DOI] [PubMed] [Google Scholar]
  4. Friedberg E. C. DNA repair of ultraviolet-irradiated bacteriophage T4. Photochem Photobiol. 1975 Apr;21(4):277–289. doi: 10.1111/j.1751-1097.1975.tb06670.x. [DOI] [PubMed] [Google Scholar]
  5. Gordon L. K., Haseltine W. A. Comparison of the cleavage of pyrimidine dimers by the bacteriophage T4 and Micrococcus luteus UV-specific endonucleases. J Biol Chem. 1980 Dec 25;255(24):12047–12050. [PubMed] [Google Scholar]
  6. Grantham R., Gautier C., Gouy M., Jacobzone M., Mercier R. Codon catalog usage is a genome strategy modulated for gene expressivity. Nucleic Acids Res. 1981 Jan 10;9(1):r43–r74. doi: 10.1093/nar/9.1.213-b. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  8. Lloyd R. S., Augustine M. L. Cloning and expression of the 3' portion of the T4 denV gene as a lacZ fusion gene. Mutat Res. 1986 Mar;165(2):89–100. doi: 10.1016/0167-8817(86)90064-7. [DOI] [PubMed] [Google Scholar]
  9. Lloyd R. S., Hanawalt P. C. Expression of the denV gene of bacteriophage T4 cloned in Escherichia coli. Proc Natl Acad Sci U S A. 1981 May;78(5):2796–2800. doi: 10.1073/pnas.78.5.2796. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McMillan S., Edenberg H. J., Radany E. H., Friedberg R. C., Friedberg E. C. den V gene of bacteriophage T4 codes for both pyrimidine dimer-DNA glycosylase and apyrimidinic endonuclease activities. J Virol. 1981 Oct;40(1):211–223. doi: 10.1128/jvi.40.1.211-223.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nakabeppu Y., Sekiguchi M. Physical association of pyrimidine dimer DNA glycosylase and apurinic/apyrimidinic DNA endonuclease essential for repair of ultraviolet-damaged DNA. Proc Natl Acad Sci U S A. 1981 May;78(5):2742–2746. doi: 10.1073/pnas.78.5.2742. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nakabeppu Y., Yamashita K., Sekiguchi M. Purification and characterization of normal and mutant forms of T4 endonuclease V. J Biol Chem. 1982 Mar 10;257(5):2556–2562. [PubMed] [Google Scholar]
  13. Radany E. H., Friedberg E. C. A pyrimidine dimer-DNA glycosylase activity associated with the v gene product of bacterophage T4. Nature. 1980 Jul 10;286(5769):182–185. doi: 10.1038/286182a0. [DOI] [PubMed] [Google Scholar]
  14. Radany E. H., Naumovski L., Love J. D., Gutekunst K. A., Hall D. H., Friedberg E. C. Physical mapping and complete nucleotide sequence of the denV gene of bacteriophage T4. J Virol. 1984 Dec;52(3):846–856. doi: 10.1128/jvi.52.3.846-856.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Recinos A., 3rd, Lloyd R. S. Efficient screening of recombinant DNA junctions afforded by probing with synthetic "bridge" oligonucleotides. Biochem Biophys Res Commun. 1986 Jul 31;138(2):945–952. doi: 10.1016/s0006-291x(86)80587-3. [DOI] [PubMed] [Google Scholar]
  16. Seawell P. C., Smith C. A., Ganesan A. K. den V gene of bacteriophage T4 determines a DNA glycosylase specific for pyrimidine dimers in DNA. J Virol. 1980 Sep;35(3):790–796. doi: 10.1128/jvi.35.3.790-796.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Valerie K., Henderson E. E., de Riel J. K. Expression of a cloned denV gene of bacteriophage T4 in Escherichia coli. Proc Natl Acad Sci U S A. 1985 Jul;82(14):4763–4767. doi: 10.1073/pnas.82.14.4763. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Valerie K., Henderson E. E., deRiel J. K. Identification, physical map location and sequence of the denV gene from bacteriophage T4. Nucleic Acids Res. 1984 Nov 12;12(21):8085–8096. doi: 10.1093/nar/12.21.8085. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Warner H. R., Christensen L. M., Persson M. L. Evidence that the UV endonuclease activity induced by bacteriophage T4 contains both pyrimidine dimer-DNA glycosylase and apyrimidinic/apurinic endonuclease activities in the enzyme molecule. J Virol. 1981 Oct;40(1):204–210. doi: 10.1128/jvi.40.1.204-210.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES