Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1986 Nov;168(2):1048–1050. doi: 10.1128/jb.168.2.1048-1050.1986

Secondary attachment site for bacteriophage lambda in the guaB gene of Escherichia coli.

M S Thomas, W T Drabble
PMCID: PMC213595  PMID: 2877966

Abstract

lambda gua transducing bacteriophages were used to identify and sequence the secondary attachment site for lambda in the guaB gene of Escherichia coli. The sequence matched the primary core sequence at nine positions, and a putative integrase binding-site overlapped the left core-arm junction. Recombinational crossover occurred between nucleotides -3 and +2 of the core region.

Full text

PDF
1048

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bauer C. E., Gardner J. F., Gumport R. I. Extent of sequence homology required for bacteriophage lambda site-specific recombination. J Mol Biol. 1985 Jan 20;181(2):187–197. doi: 10.1016/0022-2836(85)90084-1. [DOI] [PubMed] [Google Scholar]
  2. Craig N. L., Nash H. A. E. coli integration host factor binds to specific sites in DNA. Cell. 1984 Dec;39(3 Pt 2):707–716. doi: 10.1016/0092-8674(84)90478-1. [DOI] [PubMed] [Google Scholar]
  3. Craig N. L., Nash H. A. The mechanism of phage lambda site-specific recombination: site-specific breakage of DNA by Int topoisomerase. Cell. 1983 Dec;35(3 Pt 2):795–803. doi: 10.1016/0092-8674(83)90112-5. [DOI] [PubMed] [Google Scholar]
  4. Flamm E. L., Weisberg R. A. Primary structure of the hip gene of Escherichia coli and of its product, the beta subunit of integration host factor. J Mol Biol. 1985 May 25;183(2):117–128. doi: 10.1016/0022-2836(85)90206-2. [DOI] [PubMed] [Google Scholar]
  5. Hsu P. L., Landy A. Resolution of synthetic att-site Holliday structures by the integrase protein of bacteriophage lambda. Nature. 1984 Oct 25;311(5988):721–726. doi: 10.1038/311721a0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hsu P. L., Ross W., Landy A. The lambda phage att site: functional limits and interaction with Int protein. Nature. 1980 May 8;285(5760):85–91. doi: 10.1038/285085a0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kemper B., Jensch F., von Depka-Prondzynski M., Fritz H. J., Borgmeyer U., Mizuuchi K. Resolution of Holliday structures by endonuclease VII as observed in interactions with cruciform DNA. Cold Spring Harb Symp Quant Biol. 1984;49:815–825. doi: 10.1101/sqb.1984.049.01.092. [DOI] [PubMed] [Google Scholar]
  8. Landy A., Ross W. Viral integration and excision: structure of the lambda att sites. Science. 1977 Sep 16;197(4309):1147–1160. doi: 10.1126/science.331474. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Leong J. M., Nunes-Duby S., Oser A., Lesser C. F., Youderian P., Susskind M. M., Landy A. Site-specific recombination systems of phages phi 80 and P22: binding sites of integration host factor and recombination-induced mutations. Cold Spring Harb Symp Quant Biol. 1984;49:707–714. doi: 10.1101/sqb.1984.049.01.080. [DOI] [PubMed] [Google Scholar]
  10. Mizuuchi M., Mizuuchi K. Integrative recombination of bacteriophage lambda: extent of the DNA sequence involved in attachment site function. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3220–3224. doi: 10.1073/pnas.77.6.3220. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mizuuchi M., Mizuuchi K. The extent of DNA sequence required for a functional bacterial attachment site of phage lambda. Nucleic Acids Res. 1985 Feb 25;13(4):1193–1208. doi: 10.1093/nar/13.4.1193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Pinkham J. L., Platt T., Enquist L. W., Weisberg R. A. The secondary attachment site for bacteriophage lambda in the proA/B gene of Escherichia coli. J Mol Biol. 1980 Dec 25;144(4):587–592. doi: 10.1016/0022-2836(80)90339-3. [DOI] [PubMed] [Google Scholar]
  13. Ross W., Landy A. Patterns of lambda Int recognition in the regions of strand exchange. Cell. 1983 May;33(1):261–272. doi: 10.1016/0092-8674(83)90355-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sanger F., Coulson A. R., Barrell B. G., Smith A. J., Roe B. A. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. J Mol Biol. 1980 Oct 25;143(2):161–178. doi: 10.1016/0022-2836(80)90196-5. [DOI] [PubMed] [Google Scholar]
  15. Sanger F., Coulson A. R., Hong G. F., Hill D. F., Petersen G. B. Nucleotide sequence of bacteriophage lambda DNA. J Mol Biol. 1982 Dec 25;162(4):729–773. doi: 10.1016/0022-2836(82)90546-0. [DOI] [PubMed] [Google Scholar]
  16. Shimada K., Fukumaki Y., Takagi Y. Expression of the guanine operon of Escherichia coli as analyzed by bacteriophage lambda induced mutations. Mol Gen Genet. 1976 Aug 19;147(2):203–208. doi: 10.1007/BF00267572. [DOI] [PubMed] [Google Scholar]
  17. Shimada K., Weisberg R. A., Gottesman M. E. Prophage lambda at unusual chromosomal locations. I. Location of the secondary attachment sites and the properties of the lysogens. J Mol Biol. 1972 Feb 14;63(3):483–503. doi: 10.1016/0022-2836(72)90443-3. [DOI] [PubMed] [Google Scholar]
  18. Thomas M. S., Drabble W. T. Molecular cloning and characterisation of the gua regulatory region of Escherichia coli K12. Mol Gen Genet. 1984;195(1-2):238–245. doi: 10.1007/BF00332753. [DOI] [PubMed] [Google Scholar]
  19. Thomas M. S., Drabble W. T. Nucleotide sequence and organisation of the gua promoter region of Escherichia coli. Gene. 1985;36(1-2):45–53. doi: 10.1016/0378-1119(85)90068-x. [DOI] [PubMed] [Google Scholar]
  20. Tiedeman A. A., Smith J. M. Nucleotide sequence of the guaB locus encoding IMP dehydrogenase of Escherichia coli K12. Nucleic Acids Res. 1985 Feb 25;13(4):1303–1316. doi: 10.1093/nar/13.4.1303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Yin S., Bushman W., Landy A. Interaction of the lambda site-specific recombination protein Xis with attachment site DNA. Proc Natl Acad Sci U S A. 1985 Feb;82(4):1040–1044. doi: 10.1073/pnas.82.4.1040. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. de Massy B., Studier F. W., Dorgai L., Appelbaum E., Weisberg R. A. Enzymes and sites of genetic recombination: studies with gene-3 endonuclease of phage T7 and with site-affinity mutants of phage lambda. Cold Spring Harb Symp Quant Biol. 1984;49:715–726. doi: 10.1101/sqb.1984.049.01.081. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES