Abstract
Three mutant pairs of bacterial viruses T2r+ and T2r, T4r+ and T4r, T6r+ and T6r, have been concentrated by differential centrifugation and analyzed. Under conditions of prolonged lysis of organisms grown on broth-agar, the virus concentrates frequently contained phosphorus (P) other than that contained in desoxyribose nucleic acid (DNA). These concentrates reacted with antisera to the host organism, E. coli, specifically precipitating the non-DNA-P but not virus activity or DNA. The amounts of nitrogen precipitated in the virus concentrates by antisera were related to their content of non-DNA-P. These viruses could be purified by differential centrifugation after such treatment to yield preparations apparently free of P other than DNA-P. It has been concluded that there is no satisfactory evidence for the presence of ribose nucleic acid as a constituent part of these viruses.
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Selected References
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- Avery O. T., Macleod C. M., McCarty M. STUDIES ON THE CHEMICAL NATURE OF THE SUBSTANCE INDUCING TRANSFORMATION OF PNEUMOCOCCAL TYPES : INDUCTION OF TRANSFORMATION BY A DESOXYRIBONUCLEIC ACID FRACTION ISOLATED FROM PNEUMOCOCCUS TYPE III. J Exp Med. 1944 Feb 1;79(2):137–158. doi: 10.1084/jem.79.2.137. [DOI] [PMC free article] [PubMed] [Google Scholar]
- COHEN S. S., ARBOGAST R. Chemical studies in host-virus interactions; a comparison of some properties of three mutant pairs of bacterial viruses, T2r and T2r, T4r and T4r, T6r and T6r. J Exp Med. 1950 Jun 1;91(6):619–636. doi: 10.1084/jem.91.6.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
- COHEN S. S., ARBOGAST R. Chemical studies in host-virus interactions; the mutual reactivation of T2r virus inactivated by ultraviolet light and the synthesis of desoxyribose nucleic acid. J Exp Med. 1950 Jun 1;91(6):637–650. doi: 10.1084/jem.91.6.637. [DOI] [PMC free article] [PubMed] [Google Scholar]
- COHEN S. S. Adaptive enzyme formation in the study of uronic acid utilization by the K-12 strain of Escherichia coli. J Biol Chem. 1949 Feb;177(2):607–619. [PubMed] [Google Scholar]
- KOZLOFF L. M., PUTNAM F. W. Biochemical studies of virus reproduction; chemical composition of Escherichia coli bacteriophage T6 and its host. J Biol Chem. 1949 Nov;181(1):207–220. [PubMed] [Google Scholar]
- KOZLOFF L. M., PUTNAM F. W. Biochemical studies of virus reproduction; chemical composition of Escherichia coli bacteriophage T6 and its host. J Biol Chem. 1949 Nov;181(1):207–220. [PubMed] [Google Scholar]
- Kabat E. A., Furth J. CHEMICAL AND IMMUNOLOGICAL STUDIES ON THE AGENT PRODUCING LEUKOSIS AND SARCOMA OF FOWLS. J Exp Med. 1940 Jan 1;71(1):55–70. doi: 10.1084/jem.71.1.55. [DOI] [PMC free article] [PubMed] [Google Scholar]
- King E. J. The colorimetric determination of phosphorus. Biochem J. 1932;26(2):292–297. doi: 10.1042/bj0260292. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Knight C. A. A SEDIMENTABLE COMPONENT OF ALLANTOIC FLUID AND ITS RELATIONSHIP TO INFLUENZA VIRUSES. J Exp Med. 1944 Aug 1;80(2):83–100. doi: 10.1084/jem.80.2.83. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Knight C. A. PRECIPITIN REACTIONS OF HIGHLY PURIFIED INFLUENZA VIRUSES AND RELATED MATERIALS. J Exp Med. 1946 Mar 31;83(4):281–294. [PMC free article] [PubMed] [Google Scholar]
- Lauffer M. A., Stanley W. M. BIOPHYSICAL PROPERTIES OF PREPARATIONS OF PR8 INFLUENZA VIRUS. J Exp Med. 1944 Dec 1;80(6):531–548. doi: 10.1084/jem.80.6.531. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Putnam F. W., Kozloff L. M. On the Origin of Virus Phosphorus. Science. 1948 Oct 8;108(2806):386–387. doi: 10.1126/science.108.2806.386. [DOI] [PubMed] [Google Scholar]