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. 1986 Dec;168(3):1066–1069. doi: 10.1128/jb.168.3.1066-1069.1986

Mapping and complementation studies of the gene for release factor 1.

M Ryden, J Murphy, R Martin, L Isaksson, J Gallant
PMCID: PMC213603  PMID: 3782033

Abstract

In Escherichia coli the release factor 1 protein (RF1) recognizes and terminates translation at UAG and UAA codons. Using the technique of ColE1 plasmid integration in polA strains, we have mapped the cloned gene for RF1 to 27 min on the E. coli chromosome. This is the same location as that of the uar gene in which temperature-sensitive mutations increase the suppression of UAG and UAA alleles. In this study we proved that the uar mutation lies in the gene for RF1 by complementation of the uar phenotype with plasmids carrying the RF1 gene and by cloning the uar allele onto the RF1 plasmid by means of homologous recombination. In addition, complementation and P1 mapping data suggest that sueB is also a mutation in the same position as the RF1 gene. We propose that the gene for RF1 be named prfA after protein release factor.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bochner B. R., Huang H. C., Schieven G. L., Ames B. N. Positive selection for loss of tetracycline resistance. J Bacteriol. 1980 Aug;143(2):926–933. doi: 10.1128/jb.143.2.926-933.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chang A. C., Cohen S. N. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol. 1978 Jun;134(3):1141–1156. doi: 10.1128/jb.134.3.1141-1156.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Coulondre C., Miller J. H. Genetic studies of the lac repressor. III. Additional correlation of mutational sites with specific amino acid residues. J Mol Biol. 1977 Dec 15;117(3):525–567. doi: 10.1016/0022-2836(77)90056-0. [DOI] [PubMed] [Google Scholar]
  4. Davidoff-Abelson R., Mindich L. A mutation that increases the activity of nonsense suppressors in Escherichia coli. Mol Gen Genet. 1978 Feb 16;159(2):161–169. doi: 10.1007/BF00270889. [DOI] [PubMed] [Google Scholar]
  5. Gross J., Gross M. Genetic analysis of an E. coli strain with a mutation affecting DNA polymerase. Nature. 1969 Dec 20;224(5225):1166–1168. doi: 10.1038/2241166a0. [DOI] [PubMed] [Google Scholar]
  6. Johnson E. J., Cohen G. N., Saint-Girons I. Threonyl-transfer ribonucleic acid synthetase and the regulation of the threonine operon in Escherichia coli. J Bacteriol. 1977 Jan;129(1):66–70. doi: 10.1128/jb.129.1.66-70.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Menninger J. R., Caplan A. B., Gingrich P. K., Atherly A. G. Tests of the ribosome editor hypothesis. II. Relaxed (relA) and stringent (relA+) E. coli differ in rates of dissociation of peptidyl-tRNA from ribosomes. Mol Gen Genet. 1983;190(2):215–221. doi: 10.1007/BF00330642. [DOI] [PubMed] [Google Scholar]
  8. Miller J. H., Coulondre C., Farabaugh P. J. Correlation of nonsense sites in the lacI gene with specific codons in the nucleotide sequence. Nature. 1978 Aug 24;274(5673):770–775. doi: 10.1038/274770a0. [DOI] [PubMed] [Google Scholar]
  9. Oeschger M. P., Oeschger N. S., Wiprud G. T., Woods S. L. High efficiency temperature-sensitive amber suppressor strains of Escherichia coli K12: isolation of strains with suppressor-enhancing mutations. Mol Gen Genet. 1980;177(4):545–552. doi: 10.1007/BF00272662. [DOI] [PubMed] [Google Scholar]
  10. Oeschger M. P., Wiprud G. T. High efficiency temperature-sensitive amber suppressor strains of Escherichia coli K12: construction and characterization of recombinant strains with suppressor-enhancing mutations. Mol Gen Genet. 1980;178(2):293–299. doi: 10.1007/BF00270475. [DOI] [PubMed] [Google Scholar]
  11. Raftery L. A., Egan J. B., Cline S. W., Yarus M. Defined set of cloned termination suppressors: in vivo activity of isogenetic UAG, UAA, and UGA suppressor tRNAs. J Bacteriol. 1984 Jun;158(3):849–859. doi: 10.1128/jb.158.3.849-859.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rydén S. M., Isaksson L. A. A temperature-sensitive mutant of Escherichia coli that shows enhanced misreading of UAG/A and increased efficiency for some tRNA nonsense suppressors. Mol Gen Genet. 1984;193(1):38–45. doi: 10.1007/BF00327411. [DOI] [PubMed] [Google Scholar]
  13. Silver P., Wickner W. Genetic mapping of the Escherichia coli leader (signal) peptidase gene (lep): a new approach for determining the map position of a cloned gene. J Bacteriol. 1983 May;154(2):569–572. doi: 10.1128/jb.154.2.569-572.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sullivan M. A., Bock R. M. Isolation and characterization of antisuppressor mutations in Escherichia coli. J Bacteriol. 1985 Jan;161(1):377–384. doi: 10.1128/jb.161.1.377-384.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Weiss R. B., Murphy J. P., Gallant J. A. Genetic screen for cloned release factor genes. J Bacteriol. 1984 Apr;158(1):362–364. doi: 10.1128/jb.158.1.362-364.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

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