Abstract
As a preliminary to the study of desoxyribonuclease elaboration by resting cells, 34 strains of streptococci were examined for their capacity to produce desoxyribonuclease in broth cultures. The largest amounts of enzyme were found to be produced by strains belonging to Lancefield group A and by certain strains belonging to groups C and D. Many strains, especially those belonging to other groups, produced little or no enzyme. Washed cocci of certain strains elaborated desoxyribonuclease extracellularly upon suspending them in a solution containing an energy source, phosphate and magnesium ions. When any one of these factors was omitted, no enzyme was produced. The appearance of extracellular desoxyribonuclease was found to be inhibited by a variety of enzyme poisons, and this and other findings indicate that desoxyribonuclease is synthesized in the resting cell system. Cocci were found to contain a substance which partially inhibits the desoxyribonuclease formed by group A strains but which fails to inhibit the desoxyribonuclease formed by strains belonging to groups B and C or to inhibit the desoxyribonuclease derived from yeast, barley, and pancreas. The inhibitor of group A desoxyribonuclease has been identified as streptococcal ribonucleic acid. Preparations of ribonucleic acid derived from yeast differed from streptococcal ribonucleic acid in failing to inhibit the group A desoxyribonuclease.
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Selected References
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- BERNHEIMER A. W. Formation of a bacterial toxin (streptolysin S) by resting cells. J Exp Med. 1949 Nov;90(5):373–392. doi: 10.1084/jem.90.5.373. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BERNHEIMER A. W., RODBART M. The effect of nucleic acids and of carbohydrates on the formation of streptolysin. J Exp Med. 1948 Aug;88(2):149–168. doi: 10.1084/jem.88.2.149. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DABROWSKA W., COOPER E. J., LASKOWSKI M. A specific inhibitor for desoxyribonuclease. J Biol Chem. 1949 Feb;177(2):991–991. [PubMed] [Google Scholar]
- McCARTY M. The occurrence of nucleases in culture filtrates of group A hemolytic streptococci. J Exp Med. 1948 Aug;88(2):181–188. doi: 10.1084/jem.88.2.181. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mirsky A. E., Pollister A. W. Nucleoproteins of Cell Nuclei. Proc Natl Acad Sci U S A. 1942 Sep;28(9):344–352. doi: 10.1073/pnas.28.9.344. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ZAMENHOF S., CHARGAFF F. Studies on the desoxypentose nuclease of yeast and its specific cellular regulation. J Biol Chem. 1949 Sep;180(2):727–740. [PubMed] [Google Scholar]