Abstract
Substances possessing the same histochemical properties as the ceroid in cirrhotic livers of rats fed choline-deficient diets have been prepared from various unsaturated fats, fatty acids and their esters by autoxidation but could not be obtained from hydrocarbons or saturated fats or fatty acids. The formation of ceroid-like substances occurred first on surfaces or at interfaces in the reaction mixtures. It was inhibited by antioxidants and was accelerated by the addition of tissues, blood cells, erythrocytic stroma, or hemoglobin, by emulsification, by increasing the surface exposed to the air, and by increasing the temperature. Histochemical studies provided much evidence that the following properties of ceroid might be attributed to the products of the autoxidation of unsaturated lipids: insolubility in organic solvents, sudanophilia, yellowing by concentrated nitric acid, positive periodic acid-Schiff's reaction, basophilia, acid fastness, positive hernofuscin reaction, and reduction of diammine silver carbonate and alkaline potassium permanganate. The normal reactivity of cells or tissues embedded in ceroid was effectively masked by the pigment, apparently, initially at least, by preventing the reagents' gaining access to them. It is suggested that the iron sometimes demonstrated in ceroid may be that of blood cells or tissue fragments incompletely masked by the ceroid. It is concluded that whenever conditions are such that unsaturated fats accumulate in tissues to such an extent that a relative lack of biological anti-oxidants results, autoxidation of the fats and their conversion to ceroid pigment are favored, and that ceroid and the lipofuscin pigment of vitamin E deficiency may be fundamentally similar.
Full Text
The Full Text of this article is available as a PDF (1.1 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Blumberg H., McCollum E. V. THE PREVENTION BY CHOLINE OF LIVER CIRRHOSIS IN RATS ON HIGH FAT, LOW PROTEIN DIETS. Science. 1941 Jun 20;93(2425):598–599. doi: 10.1126/science.93.2425.598. [DOI] [PubMed] [Google Scholar]
- CAIN A. J. On the significance of the plasmal reaction. Q J Microsc Sci. 1949 Mar;90(1):75–86. [PubMed] [Google Scholar]
- Endicott K. M., Lillie R. D. Ceroid, the Pigment of Dietary Cirrhosis of Rats: Its Characteristics and Its Differentiation from Hemofuscin. Am J Pathol. 1944 Jan;20(1):149–153. [PMC free article] [PubMed] [Google Scholar]
- GLAVIND J., GRANADOS H. A histochemical method for the demonstration of fat peroxides. Experientia. 1949 Feb 15;5(2):84–84. doi: 10.1007/BF02153741. [DOI] [PubMed] [Google Scholar]
- GYORGY P., GOLDBLATT H. Further observations on the production and prevention of dietary hepatic injury in rats. J Exp Med. 1949 Feb;89(2):245–268. doi: 10.1084/jem.89.2.245. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GYORGY P., GOLDBLATT W. Treatment of experimental dietary cirrhosis of the liver in rats. J Exp Med. 1949 Jul;90(1):73–84. doi: 10.1084/jem.90.1.73. [DOI] [PMC free article] [PubMed] [Google Scholar]
- György P., Goldblatt H. OBSERVATIONS ON THE CONDITIONS OF DIETARY HEPATIC INJURY (NECROSIS, CIRRHOSIS) IN RATS. J Exp Med. 1942 Apr 1;75(4):355–368. doi: 10.1084/jem.75.4.355. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gōmōri G. Microtechnical Demonstration of Iron: A Criticism of its Methods. Am J Pathol. 1936 Sep;12(5):655–664.1. [PMC free article] [PubMed] [Google Scholar]
- HARTROFT W. S. In vitro and in vivo production of a ceroidlike substance from erythrocytes and certain lipids. Science. 1951 Jun 15;113(2946):673–674. doi: 10.1126/science.113.2946.673. [DOI] [PubMed] [Google Scholar]
- McMANUS J. F. A. The periodic acid routing applied to the kidney. Am J Pathol. 1948 May;24(3):643–653. [PMC free article] [PubMed] [Google Scholar]
- Pappenheimer A. M., Victor J. "Ceroid" Pigment in Human Tissues. Am J Pathol. 1946 Mar;22(2):395–413. [PMC free article] [PubMed] [Google Scholar]
- Victor J., Pappenheimer A. M. THE INFLUENCE OF CHOLINE, CYSTINE, AND OF alpha-TOCOPHEROL UPON THE OCCURRENCE OF CEROID PIGMENT IN DIETARY CIRRHOSIS OF RATS. J Exp Med. 1945 Nov 30;82(6):375–383. [PMC free article] [PubMed] [Google Scholar]