Abstract
Sera from 81 patients with a diagnosis of paralytic or non-paralytic poliomyelitis, and from 159 individuals of similar age groups giving no history of the disease, were tested with a high titered, complement-fixing poliomyelitis antigen of Type 2 (Lansing-like). The antigen consisted of brain tissue from newborn mice injected with the MEF1 strain of virus as previously adapted to these animals. The presence or absence of Type 2 neutralizing antibody in the sera under test was found not to affect the complement fixation. Positive reactions were obtained with 57 per cent of the sera deriving from non-paralytic patients and in 70 per cent from paralytics, when the specimens were tested at a dilution of 1:16. The complement-fixing antibody was often present in highest titer as early as 24 hours after the onset of poliomyelitis, and in almost all instances within 7 days. In about half of the patients a 4-fold or greater drop in titer occurred within 3 months, with little or no change in the others. The incidence of titers of 1:16 or higher with the control sera varied with the season of the year at which they were procured, 3 per cent of the winter samples proving positive and 13 per cent of the summer. The tests of sera from the group of patients from whom poliomyelitis virus was recovered, disclosed no significant differences between those having the paralytic and those having the non-paralytic disease. Type 1 (Brunhilde-like) strains of virus were recovered from many of the patients yielding positive tests, although they presented no evidence of previous or concurrent infection with Type 2 virus. This finding shows that Type 1 virus can give rise in patients to Type 2 complement-fixing antibody. The application of these data to the serologic diagnosis of poliomyelitis infection in man will of necessity be limited until information is obtained on the development, persistence, and significance of complement-fixation reactions with antigens deriving from Type 1 and Type 3 poliomyelitis strains.
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Selected References
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- CASALS J. Acetone-ether extracted antigens for complement-fixation with certain neurotropic viruses. Proc Soc Exp Biol Med. 1949 Feb;70(2):339–343. doi: 10.3181/00379727-70-16921. [DOI] [PubMed] [Google Scholar]
- CASALS J., OLITSKY P. K., ANSLOW R. O. A specific complement-fixation test for infection with poliomyelitis virus. J Exp Med. 1951 Aug;94(2):123–137. doi: 10.1084/jem.94.2.123. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CASALS J., OLITSKY P. K., ANSLOW R. O. Adaptation of a Lansing strain of poliomyelitis virus to newborn mice. J Exp Med. 1951 Aug;94(2):111–121. doi: 10.1084/jem.94.2.111. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CASALS J., OLITSKY P. K., SABIN A. B. Homotypic complement-fixing antibody in monkeys infected with type 2 poliomyelitis virus by the oral route. J Exp Med. 1952 Jul;96(1):55–58. doi: 10.1084/jem.96.1.55. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CASALS J., OLITSKY P. K. The adaptation of poliomyelitis virus, Lansing strain, to newborn mice and the use of their central nervous tissues for preparation of complement-fixing antigen. Arch Gesamte Virusforsch. 1951;4(4):452–459. doi: 10.1007/BF01241165. [DOI] [PubMed] [Google Scholar]
- HAMMON W. M., ROBERTS E. C. Serum neutralizing antibodies to the infecting strain of virus in poliomyelitis patients. Proc Soc Exp Biol Med. 1948 Nov;69(2):256–258. doi: 10.3181/00379727-69-16682. [DOI] [PubMed] [Google Scholar]
- LAHELLE O. Complement-fixation test for the Lansing strain of poliomyelitis virus. Am J Hyg. 1951 Nov;54(3):391–401. doi: 10.1093/oxfordjournals.aje.a119494. [DOI] [PubMed] [Google Scholar]
- POLLARD M., HSIANG C. M., SHARP G. R. Preparation of specific complement-fixing antigens from the three prototypes of poliomyelitis viruses. Proc Soc Exp Biol Med. 1952 Jan;79(1):48–50. doi: 10.3181/00379727-79-19268. [DOI] [PubMed] [Google Scholar]
- POLLARD M. Preparation of specific complement-fixing antigen with Lansing poliomyelitis virus. Proc Soc Exp Biol Med. 1951 Nov;78(2):388–392. doi: 10.3181/00379727-78-19082. [DOI] [PubMed] [Google Scholar]
- SABIN A. B. Paralytic consequences of poliomyelitis infection in different parts of the world and in different population groups. Am J Public Health Nations Health. 1951 Oct;41(10):1215–1230. doi: 10.2105/ajph.41.10.1215. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SABIN A. B. Transitory appearance of type 2 neutralizing antibody in patients infected with type 1 poliomyelitis virus. J Exp Med. 1952 Jul;96(1):99–106. doi: 10.1084/jem.96.1.99. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STEIGMAN A. J., SABIN A. B. Antibody response of patients with poliomyelitis to virus recovered from their own alimentary tract. J Exp Med. 1949 Oct;90(4):349–372. doi: 10.1084/jem.90.4.349. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SVEDMYR A., ENDERS J. F., HOLLOWAY A. Complement fixation with Brunhilde and Lansing poliomyelitis viruses propagated in tissue culture. Proc Soc Exp Biol Med. 1952 Feb;79(2):296–300. doi: 10.3181/00379727-79-19357. [DOI] [PubMed] [Google Scholar]
- TURNER T. B., HOLLANDER D. H., BUCKLEY S., KOKKO U. P., WINSOR C. P. Age incidence and seasonal development of neutralizing antibodies to lansing poliomyelitis virus. Am J Hyg. 1950 Nov;52(3):323–347. doi: 10.1093/oxfordjournals.aje.a119427. [DOI] [PubMed] [Google Scholar]