Abstract
A technique has been described for the cultivation in vitro of normal mononuclear cells on glass slides in a liquid medium. Under these conditions the monocytes transformed into macrophages which proliferated as in ordinary tissue culture. These cultures of monocytes could be infected with tubercle bacilli. The numbers of stainable tubercle bacilli within the monocytes increased steadily in cultures infected with virulent or attenuated strains. Evidence is given to support the view that this increase in numbers of bacilli was due to intracellular multiplication. There was no evidence of intracellular bacillary multiplication in cultures infected with an avirulent strain. Tubercle bacilli multiplying within phagocytes in vitro exert a damaging effect upon the host cells. The damage was most obvious in cells infected with a virulent strain. Tubercle bacilli within phagocytes were protected against the bacteriostatic effect of streptomycin added in a concentration of 5 γ per ml. of culture medium. This permitted the use of streptomycin in infected cultures to prevent extracellular multiplication of the bacilli.
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Selected References
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- AUBERT E. Cold stain for acid-fast bacteria. Can J Public Health. 1950 Jan;41(1):31–31. [PubMed] [Google Scholar]
- BRIEGER E. M. The host parasite relationship in tuberculous infection. Tubercle. 1949 Nov;30(11):242–253. doi: 10.1016/s0041-3879(49)80071-7. [DOI] [PubMed] [Google Scholar]
- HANKS J. H., WALLACE R. E. Relation of oxygen and temperature in the preservation of tissues by refrigeration. Proc Soc Exp Biol Med. 1949 Jun;71(2):196–200. doi: 10.3181/00379727-71-17131. [DOI] [PubMed] [Google Scholar]
- Lewis P. A., Sanderson E. S. THE HISTOLOGICAL EXPRESSION OF THE NATURAL RESISTANCE OF RABBITS TO INFECTION WITH HUMAN AND BOVINE TYPE TUBERCLE BACILLI. J Exp Med. 1927 Jan 31;45(2):291–304. doi: 10.1084/jem.45.2.291. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lurie M. B., Collaboration of Peter Zappasodi STUDIES ON THE MECHANISM OF IMMUNITY IN TUBERCULOSIS : THE FATE OF TUBERCLE BACILLI INGESTED BY MONONUCLEAR PHAGOCYTES DERIVED FROM NORMAL AND IMMUNIZED ANIMALS. J Exp Med. 1942 Mar 1;75(3):247–268. doi: 10.1084/jem.75.3.247. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lurie M. B. THE FATE OF HUMAN AND BOVINE TUBERCLE BACILLI IN VARIOUS ORGANS OF THE RABBIT. J Exp Med. 1928 Jul 31;48(2):155–182. doi: 10.1084/jem.48.2.155. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Martin S. P., Pierce C. H., Middlebrook G., Dubos R. J. THE EFFECT OF TUBERCLE BACILLI ON THE POLYMORPHONUCLEAR LEUCOCYTES OF NORMAL ANIMALS. J Exp Med. 1950 Mar 31;91(4):381–392. doi: 10.1084/jem.91.4.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Porter K. R., Claude A., Fullam E. F. A STUDY OF TISSUE CULTURE CELLS BY ELECTRON MICROSCOPY : METHODS AND PRELIMINARY OBSERVATIONS. J Exp Med. 1945 Mar 1;81(3):233–246. doi: 10.1084/jem.81.3.233. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SUTER W. E., DUBOS R. J. Variability of BCG strains (Bacillus Calmette-Guérin). J Exp Med. 1951 Jun;93(6):559–572. doi: 10.1084/jem.93.6.559. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sabin F. R., Doan C. A. THE RELATION OF MONOCYTES AND CLASMATOCYTES TO EARLY INFECTION IN RABBITS WITH BOVINE TUBERCLE BACILLI. J Exp Med. 1927 Sep 30;46(4):627–644. doi: 10.1084/jem.46.4.627. [DOI] [PMC free article] [PubMed] [Google Scholar]