Abstract
Light-induced ATP synthesis was studied in intact cells and chromatophores of Erythrobacter sp. strain OCh114. ATP synthesis was measured by both the pH method and the luciferin-luciferase luminescence method. The rate of ATP synthesis was moderate (a typical value of 0.65 mol of ATP per mol of bacteriochlorophyll per min), and synthesis was inhibited by antimycin A. ATP was synthesized under illumination only under aerobic conditions and not under anaerobic conditions. This characteristic was similar to that of other light-induced energy transduction processes in this bacterial species, such as oxidation of reaction center, oxidation of cytochrome c551, and translocation of H+, which were not observed under anaerobic conditions. This phenomenon was reconciled with the fact that the Erythrobacter sp. could not grow anaerobically even in the light. The characteristics of oxidative phosphorylation and ATP hydrolysis were also investigated. The respiratory ratio of chromatophores was 2.3. Typical rates of oxidative phosphorylation by NADH and by succinate were 2.9 mol of ATP per mol of bacteriochlorophyll per min (P/O = 0.22) and 1.1 mol of ATP per mol of bacteriochlorophyll per min (P/O = 0.19), respectively. A typical rate of ATP hydrolysis was 0.25 mol of ATP per mol of bacteriochlorophyll per min in chromatophores. ATPase and adenylate kinase are also involved in the metabolism of adenine nucleotides in this bacterium.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baccarini Melandri A., Zannoni D., Melandri B. A. Energy transduction in photosynthetic bacteria. VI. Respiratory sites of energy conservation in membranes from dark-grown cells of Rhodopseudomonas capsulata. Biochim Biophys Acta. 1973 Sep 26;314(3):298–311. doi: 10.1016/0005-2728(73)90114-x. [DOI] [PubMed] [Google Scholar]
- Leps W. T., Ensign J. C. Adenosine triphosphate pool levels and endogenous metabolism in Arthrobacter crystallopoietes during growth and starvation. Arch Microbiol. 1979 Jul;122(1):61–67. doi: 10.1007/BF00408047. [DOI] [PubMed] [Google Scholar]
- NISHIMURA M., ITO T., CHANCE B. Studies on bacterial photophosphorylation. III. A sensitive and rapid method of determination of photophosphorylation. Biochim Biophys Acta. 1962 May 7;59:177–182. [PubMed] [Google Scholar]
- Sato K. Bacteriochlorophyll formation by facultative methylotrophs, Protaminobacter ruber and Pseudomonas AM 1. FEBS Lett. 1978 Jan 15;85(2):207–210. doi: 10.1016/0014-5793(78)80456-6. [DOI] [PubMed] [Google Scholar]
- Saunders V. A., Jones O. T. Oxidative phosphorylation and effects of aerobic conditions on Rhodopseudomonas viridis. Biochim Biophys Acta. 1973 Jun 28;305(3):581–589. doi: 10.1016/0005-2728(73)90077-7. [DOI] [PubMed] [Google Scholar]
- Thore A., Keister D. L., Shavit N., San Pietro A. Effects of antibiotics on ion transport and photophosphorylation in Rhodospirillum rubrum chromatophores. Biochemistry. 1968 Oct;7(10):3499–3507. doi: 10.1021/bi00850a026. [DOI] [PubMed] [Google Scholar]