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. 1952 Oct 31;96(5):465–476. doi: 10.1084/jem.96.5.465

STUDIES ON THE EXTRACELLULAR CULTIVATION OF AN INTRACELLULAR PARASITE (AVIAN MALARIA)

II. THE EFFECTS OF MALATE AND OF COENZYME A CONCENTRATES

William Trager 1
PMCID: PMC2136161  PMID: 13000057

Abstract

The extracellular survival and development in vitro of the erythrocytic stages of Plasmodium lophurae were favored by the addition to the culture medium of l-malic acid and concentrates rich in coenzyme A. In a concentrated extract of duck erythrocytes supplemented with these two substances in addition to adenosinetriphosphate, sodium pyruvate, and certain other materials of like nature, only 5 to 10 per cent of the extracellular parasites had become abnormal after 3 days of cultivation.

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Selected References

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  1. TRAGER W. Studies on the extracellular cultivation of an intracellular parasite (avian malaria). I. Development of the organisms in erythrocyte extracts, and the favoring effect of adenosinetriphosphate. J Exp Med. 1950 Oct 1;92(4):349–366. doi: 10.1084/jem.92.4.349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Trager W. FURTHER STUDIES ON THE SURVIVAL AND DEVELOPMENT IN VITRO OF A MALARIAL PARASITE. J Exp Med. 1943 May 1;77(5):411–420. doi: 10.1084/jem.77.5.411. [DOI] [PMC free article] [PubMed] [Google Scholar]

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