Abstract
The lysis of group A hemolytic streptococci by extracellular enzymes of Streptomyces albus has been studied. The most favorable material for fractionation of the lytic enzymes was obtained by surface growth on shallow layers of liquid medium containing an acid hydrolysate of casein, glucose, and salts. The results of fractionation experiments show that the potent proetolytic enzyme of Streptomyces albus is not able to lyse group A streptococci, and that the initiation of lysis is dependent upon the action of a second, non-proteolytic enzyme. The nature of the non-proteolytic enzyme has not been determined. It does not appear to be a ribonuclease or a lysozyme-like enzyme.
Full Text
The Full Text of this article is available as a PDF (790.4 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- LONGFELLOW D., CARY S. G., KUHN L. R. Use of Maxted's streptomyces filtrate for rapidly extracting group carbohydrates from hemolytic streptococci. Am J Clin Pathol. 1949 Sep;19(9):880–882. doi: 10.1093/ajcp/19.9_ts.880. [DOI] [PubMed] [Google Scholar]
- Lancefield R. C., Dole V. P. THE PROPERTIES OF T ANTIGENS EXTRACTED FROM GROUP A HEMOLYTIC STREPTOCOCCI. J Exp Med. 1946 Oct 31;84(5):449–471. doi: 10.1084/jem.84.5.449. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lancefield R. C. THE ANTIGENIC COMPLEX OF STREPTOCOCCUS HAEMOLYTICUS : I. DEMONSTRATION OF A TYPE-SPECIFIC SUBSTANCE IN EXTRACTS OF STREPTOCOCCUS HAEMOLYTICUS. J Exp Med. 1928 Jan 1;47(1):91–103. doi: 10.1084/jem.47.1.91. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MUGGLETON P. W., WEBB M. The exocellular bacteriolytic system of soil Actinomyces. II. A further investigation of the lytic system. Biochim Biophys Acta. 1952 Apr;8(4):431–441. doi: 10.1016/0006-3002(52)90069-3. [DOI] [PubMed] [Google Scholar]
- TAI T. Y., van HEYNINGEN W. E. Bacteriolysis by a species of Streptomyces. J Gen Microbiol. 1951 Feb;5(1):110–120. doi: 10.1099/00221287-5-1-110. [DOI] [PubMed] [Google Scholar]