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. 1953 Jan 1;97(1):77–90. doi: 10.1084/jem.97.1.77

THE PROTECTION OF INTRACELLULAR BRUCELLA AGAINST THERAPEUTIC AGENTS AND THE BACTERICIDAL ACTION OF SERUM

James M Shaffer 1, Carrell J Kucera 1, Wesley W Spink 1
PMCID: PMC2136185  PMID: 13022864

Abstract

A method for the in vitro study of intracellular brucella has been described. Exudative leukocytes containing intracellular brucella have been maintained in vitro in a synthetic tissue culture medium or in human or animal serum. Intracellular brucella are protected in vitro against the lethal action of therapeutic agents or the bactericidal action of serum. This protection of intracellular brucella is dependent upon the presence of an intact, viable host cell. None of the currently available therapeutic agents, whether used alone or in combinations, were capable of killing all intracellular brucella in vitro in 24 hours. A remarkable protection of intracellular brucella against streptomycin has been demonstrated. The most effective reduction in the number of viable intracellular brucella was accomplished by exposure of the host cells to streptomycin plus aureomycin, terramycin, or chloramphenicol. The available evidence suggests that the ability of brucella to localize and remain viable within the cells of an infected host is an important biologic factor in establishing and perpetuating brucella infections, despite therapeutic measures or the operation of the host's humoral defense mechanisms. Reduction of neotetrazolium by leukocytes and brucella in vitro provides a method for assessing the metabolic status of the host cell, but does not discriminate with any degree of certainty a viable from a non-viable intracellular organism.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRAUDE A. I. Studies in the pathology and pathogenesis of experimental brucellosis. II. The formation of the hepatic granuloma and its evolution. J Infect Dis. 1951 Jul-Aug;89(1):87–94. doi: 10.1093/infdis/89.1.87. [DOI] [PubMed] [Google Scholar]
  2. Goodpasture E. W. Concerning the Pathogenesis of Typhoid Fever. Am J Pathol. 1937 Mar;13(2):175–186.5. [PMC free article] [PubMed] [Google Scholar]
  3. HEILMAN F. R. The effect of combined treatment with aureomycin and dihydrostreptomycin on brucella infections in mice. Proc Staff Meet Mayo Clin. 1949 Mar 16;24(6):133–137. [PubMed] [Google Scholar]
  4. HERRELL W. E., BARBER T. E. A new method for treatment of brucellosis. J Am Med Assoc. 1950 Oct 14;144(7):519–524. doi: 10.1001/jama.1950.02920070007003. [DOI] [PubMed] [Google Scholar]
  5. KELLY E. H., GORELICK A. N. Therapy of experimental brucellosis. J Infect Dis. 1952 May-Jun;90(3):249–257. doi: 10.1093/infdis/90.3.249. [DOI] [PubMed] [Google Scholar]
  6. MACKANESS G. B., SMITH N. The action of isoniazid (isonicotinic acid hydrazide) on intracellular tubercle bacilli. Am Rev Tuberc. 1952 Aug;66(2):125–133. doi: 10.1164/art.1952.66.2.125. [DOI] [PubMed] [Google Scholar]
  7. MACKANESS G. B. The action of drugs on intracellular tubercle bacilli. J Pathol Bacteriol. 1952 Jul;64(3):429–446. doi: 10.1002/path.1700640302. [DOI] [PubMed] [Google Scholar]
  8. MAGOFFIN R. L., SPINK W. W. The protection of intracellular brucella against streptomycin alone and in combination with other antibiotics. J Lab Clin Med. 1951 Jun;37(6):924–930. [PubMed] [Google Scholar]
  9. Martin S. P., Pierce C. H., Middlebrook G., Dubos R. J. THE EFFECT OF TUBERCLE BACILLI ON THE POLYMORPHONUCLEAR LEUCOCYTES OF NORMAL ANIMALS. J Exp Med. 1950 Mar 31;91(4):381–392. doi: 10.1084/jem.91.4.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rous P., Jones F. S. THE PROTECTION OF PATHOGENIC MICROORGANISMS BY LIVING TISSUE CELLS. J Exp Med. 1916 May 1;23(5):601–612. doi: 10.1084/jem.23.5.601. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. SMITH F. E. Tetrazolium salt. Science. 1951 Jun 29;113(2948):751–754. doi: 10.1126/science.113.2948.751-a. [DOI] [PubMed] [Google Scholar]
  12. SPINK W. W., HALL W. H., MAGOFFIN R. Follow-up study of therapy in forty-eight culturally proved cases of brucellosis; streptomycin and sulfadiazine, aureomycin, and chloramphenicol (chloromycetin). AMA Arch Intern Med. 1951 Oct;88(4):419–432. doi: 10.1001/archinte.1951.03810100003001. [DOI] [PubMed] [Google Scholar]
  13. SUTER E. Multiplication of tubercle bacilli within phagocytes cultivated in vitro, and effect of streptomycin and isonicotinic acid hydrazide. Am Rev Tuberc. 1952 Jun;65(6):775–776. doi: 10.1164/art.1952.65.6.775. [DOI] [PubMed] [Google Scholar]
  14. WERNER C. A., KNIGHT V. The suppressive effect of antimicrobial drugs on Brucella melitensis infection in mice. J Immunol. 1950 Nov;65(5):509–514. [PubMed] [Google Scholar]

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