Abstract
Structural changes were regularly observed in Brown-Pearce rabbit carcinoma cells that had been brought into contact, in the presence of complement, with rabbit serum containing an antibody that reacts specifically with a distinctive sedimentable constituent of the carcinoma cells. The cellular changes appeared rapidly and were accompanied by an equally rapid loss of viability. The structural changes, as disclosed by histological and cytological methods, including phase contrast microscopy, were described in detail and illustrated. Essentially they were characterized by the entrance of considerable quantities of fluid into the cell, together with swelling and disorganization of its cytoplasm, vesiculation and rupture of the bulk of its cytoplasmic particles, and rapid and virtually complete loss of cytoplasmic basophilism. The plasma membranes, though stretched thin about the swollen cytoplasm of the altered cells, and otherwise changed also, remained unruptured during many hours' observation. The nuclei of the altered cells, however, remained relatively un-changed, their membranes persisting unruptured and their sap remaining wholly transparent and undiminished in amount for long periods, while within it the chromatin retained its staining properties, though sometimes becoming clumped and marginated; by contrast, the nucleoli of the treated cells promptly shrank and lost much of their affinity for the basic dyes. The experimental conditions under which the structural changes became manifest were given in detail, together with an analysis of the findings and brief mention of certain of their implications for cytology and immunology.
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- BORSOOK H. Protein turnover and incorporation of labeled amino acids into tissue proteins in vivo and in vitro. Physiol Rev. 1950 Apr;30(2):206–219. doi: 10.1152/physrev.1950.30.2.206. [DOI] [PubMed] [Google Scholar]
- Bartholomew J. W., Umbreit W. W. Ribonucleic Acid and the Gram Stain. J Bacteriol. 1944 Nov;48(5):567–578. doi: 10.1128/jb.48.5.567-578.1944. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CRAIGIE J. Further observations on tumour cells in the paramorphic or dormant resistant state. J Pathol Bacteriol. 1952 Jan;64(1):251–252. [PubMed] [Google Scholar]
- CRAIGIE J., LIND P. E., HAYWARD M. E., BEGG A. M. Preliminary observations on a "dormant" state of sarcoma cells with special reference to resistance to freezing and drying. J Pathol Bacteriol. 1951 Jan;63(1):177–178. [PubMed] [Google Scholar]
- Dubos R. J. THE AUTOLYTIC SYSTEM OF PNEUMOCOCCI. J Exp Med. 1937 May 31;65(6):873–883. doi: 10.1084/jem.65.6.873. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Friedewald W. F., Kidd J. G. INDUCED ANTIBODIES THAT REACT IN VITRO WITH SEDIMENTABLE CONSTITUENTS OF NORMAL AND NEOPLASTIC TISSUE CELLS : PRESENCE OF THE ANTIBODIES IN THE BLOOD OF RABBITS CARRYING VARIOUS TRANSPLANTED CANCERS. J Exp Med. 1945 Jul 1;82(1):21–39. doi: 10.1084/jem.82.1.21. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kidd J. G. A DISTINCTIVE SUBSTANCE ASSOCIATED WITH THE BROWN-PEARCE RABBIT CARCINOMA : I. PRESENCE AND SPECIFICITY OF THE SUBSTANCE AS DETERMINED BY SERUM REACTIONS. J Exp Med. 1940 Feb 29;71(3):335–350. doi: 10.1084/jem.71.3.335. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kidd J. G., Rous P. A TRANSPLANTABLE RABBIT CARCINOMA ORIGINATING IN A VIRUS-INDUCED PAPILLOMA AND CONTAINING THE VIRUS IN MASKED OR ALTERED FORM. J Exp Med. 1940 May 31;71(6):813–838. doi: 10.1084/jem.71.6.813. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kidd J. G. SUPPRESSION OF GROWTH OF BROWN-PEARCE TUMOR CELLS BY A SPECIFIC ANTIBODY : WITH A CONSIDERATION OF THE NATURE OF THE REACTING CELL CONSTITUENT. J Exp Med. 1946 Feb 28;83(3):227–250. [PMC free article] [PubMed] [Google Scholar]
- Mackenzie I., Kidd J. G. INCIDENCE AND SPECIFICITY OF THE ANTIBODY FOR A DISTINCTIVE CONSTITUENT OF THE BROWN-PEARCE TUMOR. J Exp Med. 1945 Jul 1;82(1):41–63. doi: 10.1084/jem.82.1.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mayer M. M., Osler A. G., Bier O. G., Heidelberger M. THE ACTIVATING EFFECT OF MAGNESIUM AND OTHER CATIONS ON THE HEMOLYTIC FUNCTION OF COMPLEMENT. J Exp Med. 1946 Nov 30;84(6):535–548. doi: 10.1084/jem.84.6.535. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Opie E. L. NORMAL STRUCTURE AND DEGENERATIVE CHANGES OF THE CYTOPLASM OF LIVER CELLS AND OF TUMOR CELLS DERIVED FROM THEM. J Exp Med. 1947 Mar 31;85(4):339–346. doi: 10.1084/jem.85.4.339. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Witebsky E., Neter E. PRIMARY SERUM TOXICITY AS DEMONSTRATED BY THE CHICKEN EMBRYO. J Exp Med. 1935 Mar 31;61(4):489–499. doi: 10.1084/jem.61.4.489. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ZOLLINGER H. U. Cytologic studies with the phase microscope; morphologic changes associated with the death of cells in vitro and in vivo. Am J Pathol. 1948 Sep;24(5):1039–1053. [PMC free article] [PubMed] [Google Scholar]