Abstract
Employing fluorescent antibodies for the detection of homologous plasma proteins in tissue sections, the distribution of plasma albumin, γ-globulin, β-lipoprotein, β1-metal-combining globulin, and fibrinogen has been studied in the tissues of infants and children. Plasma albumin, γ-globulin, and β1-metal-combining globulin were found in many cells and particularly cell nuclei, connective tissues and interstitial spaces, lymphatics, and blood vessels. β-Lipoprotein was found mostly in the nuclei of all cell types while fibrinogen was restricted largely to the lymphatic and vascular channels, connective tissues and the interstitial spaces. The widespread distribution of these plasma proteins in cells and connective tissues indicates the magnitude of the extravascular plasma protein pool which is in equilibrium with circulating plasma. Unfortunately, these results do not permit accurate localization of the sites of production of these plasma proteins, but do give some idea of their intimate relationship to the tissues.
Full Text
The Full Text of this article is available as a PDF (1.2 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- COHN M., WETTER L. R., DEUTSCH H. F. Immunological studies on egg white proteins; precipitation of chicken-ovalbumin and conalbumin by rabbit- and horse-antisera. J Immunol. 1949 Apr;61(4):283–296. [PubMed] [Google Scholar]
- COONS A. H., LEDUC E. H., KAPLAN M. H. Localization of antigen in tissue cells. VI. The fate of injected foreign proteins in the mouse. J Exp Med. 1951 Feb;93(2):173–188. doi: 10.1084/jem.93.2.173. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CRAMPTON C. F., HAUROWITZ F. The intracellular distribution in rabbit liver of injected antigens labeled with I131. Science. 1950 Sep 15;112(2907):300–302. doi: 10.1126/science.112.2907.300. [DOI] [PubMed] [Google Scholar]
- GITLIN D., DAVIDSON C. S., WETTERLOW L. H. The quantitative estimation of serum albumin in human body fluids by direct titration with specific horse at tiserum. J Immunol. 1949 Dec;63(4):415–425. [PubMed] [Google Scholar]
- GITLIN D., EDELHOCH H. A study of the reaction between human serum albumin and its homologous equine antibody through the medium of light scattering. J Immunol. 1951 Jan;66(1):67–77. [PubMed] [Google Scholar]
- GITLIN D., JANEWAY C. A. An immunochemical study of the albumins of serum, urine, ascitic fluid and edema fluid in the nephrotic syndrome. J Clin Invest. 1952 Feb;31(2):223–230. doi: 10.1172/JCI102596. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GITLIN D., LATTA H., BATCHELOR W. H., JANEWAY C. A., CUSHMAN M., MARX K. Experimental hypersensitivity in the rabbit; disappearance rates of native and labelled heterologous proteins from the serum after intravenous injection. J Immunol. 1951 Apr;66(4):451–461. [PubMed] [Google Scholar]
- GITLIN D. Use of ultraviolet absorption spectroscopy in the quantitative precipitin reaction. J Immunol. 1949 Aug;62(4):437–451. [PubMed] [Google Scholar]
- Heidelberger M., Kendall F. E. A QUANTITATIVE THEORY OF THE PRECIPITIN REACTION : III. THE REACTION BETWEEN CRYSTALLINE EGG ALBUMIN AND ITS HOMOLOGOUS ANTIBODY. J Exp Med. 1935 Oct 31;62(5):697–720. doi: 10.1084/jem.62.5.697. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MARSHALL J. M., Jr Localization of adrenocorticotropic hormone by histochemical and immunochemical methods. J Exp Med. 1951 Jul 1;94(1):21–30. doi: 10.1084/jem.94.1.21. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pappenheimer A. M., Lundgren H. P., Williams J. W. STUDIES ON THE MOLECULAR WEIGHT OF DIPHTHERIA TOXIN, ANTITOXIN, AND THEIR REACTION PRODUCTS. J Exp Med. 1940 Jan 31;71(2):247–262. doi: 10.1084/jem.71.2.247. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SOROF S., COHEN P. P. Electrophoretic studies of the soluble proteins of rabbit liver. J Biol Chem. 1951 May;190(1):303–309. [PubMed] [Google Scholar]
- STERLING K. The turnover rate of serum albumin in man as measured by I131-tagged albumin. J Clin Invest. 1951 Nov;30(11):1228–1237. doi: 10.1172/JCI102542. [DOI] [PMC free article] [PubMed] [Google Scholar]