Abstract
Treatment of radioactively labelled host cells with T1 or T2 bacteriophages induces a leakage of cellular P and S into the medium. Evidence is presented showing that this increased cell permeability is not the result of complete lysis of a small fraction of the cells, but rather is made up of contributions from all or most of the infected population. This leakage of cellular constituents exhibits the following characteristics: (a) Infection of a cell with a single virus suffices to evoke the reaction; (b) Increasing the multiplicity up to 7 to 8 virus particles per cell does not affect the extent of leakage produced; (c) Some leakage does occur at 0°C., but much less than at 37°C.; (d) Infection by T1 virus results in a smaller amount of leakage than in the case of T2, but the pattern of response to varying virus multiplicity is the same; (e) The P resulting from such leakage contains no DNA and chemically resembles that which elutes in smaller amounts from uninfected cells; (f) At 37°C. the virus-induced leakage reaction appears within a matter of seconds, and usually decreases after 2 to 3 minutes; (g) The reaction is inhibited by 0.025 M Mg++. Theoretical considerations are presented suggesting the place of this reaction in the sequence of events constituting the virus penetration reaction; its relationship to the phenomenon of lysis-from-without; and its resemblance to the leakage reaction produced by electrostatic binding of ionized compounds to cell surfaces. The existence of similar effects in avian-mammalian virus systems is noted.
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- GANDELMAN B., ZAMENHOF S., CHARGAFF E. The desoxypentose nucleic acids of three strains of Escherichia coli. Biochim Biophys Acta. 1952 Oct;9(4):399–401. doi: 10.1016/0006-3002(52)90183-2. [DOI] [PubMed] [Google Scholar]
- GAREN A., PUCK T. T. The first two steps of the invasion of host cells by bacterial viruses. II. J Exp Med. 1951 Sep;94(3):177–189. doi: 10.1084/jem.94.3.177. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HEAGY F. C. The effect of 2,4-dinitrophenol and phage T2 on Escherichia coli B. J Bacteriol. 1950 Mar;59(3):367–373. doi: 10.1128/jb.59.3.367-373.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HERSHEY A. D., CHASE M. Independent functions of viral protein and nucleic acid in growth of bacteriophage. J Gen Physiol. 1952 May;36(1):39–56. doi: 10.1085/jgp.36.1.39. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HERSHEY A. D., DIXON J., CHASE M. Nucleic acid economy in bacteria infected with bacteriophage T2. I. Purine and pyrimidine composition. J Gen Physiol. 1953 Jul;36(6):777–789. doi: 10.1085/jgp.36.6.777. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PUCK T. T. The first steps of virus invasion. Cold Spring Harb Symp Quant Biol. 1953;18:149–154. doi: 10.1101/sqb.1953.018.01.024. [DOI] [PubMed] [Google Scholar]
- PUCK T., SAGIK B. Virus and cell interaction with ion exchangers. J Exp Med. 1953 Jun;97(6):807–820. doi: 10.1084/jem.97.6.807. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SAGIK B., PUCK T., LEVINE S. Quantitative aspects of the spontaneous elution of influenza virus from red cells. J Exp Med. 1954 Mar;99(3):251–260. doi: 10.1084/jem.99.3.251. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SALTON M. R. J. The adsorption of cetyltrimethylammonium bromide by bacteria, its action in releasing cellular constituents and its bactericidal effects. J Gen Microbiol. 1951 May;5(2):391–404. doi: 10.1099/00221287-5-2-391. [DOI] [PubMed] [Google Scholar]
- WATSON J. D. The properties of x-ray inactivated bacteriophage. I. Inactivation by direct effect. J Bacteriol. 1950 Dec;60(6):697–718. doi: 10.1128/jb.60.6.697-718.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]