Abstract
Carbon-14-labeled plasma proteins given by mouth to dogs with sterile abscesses undergo decreased absorption, presumably owing to impaired digestion of protein. The turnover of plasma albumin is greatly accelerated but the globulins, excluding fibrinogen, show little change during the acute stage of the sterile inflammation. Fibrinogen shows very rapid production and utilization during acute inflammation. Large amounts of C14 are incorporated in fibrinogen within a few hours after ingestion of the labeled material. The labeled fibrinogen largely disappears within 2 to 4 days after its production. The appearance of C14 in new red cells from labeled protein or amino acid sources is reduced by inflammation—evidence of impaired synthesis. The pus of the sterile abscess contains a good deal of C14 activity which at times is as much as that found in the liver. Pus cell C14 activity per milliliter is similar after injection of labeled plasma and ingestion of labeled plasma or lysine. However, the pus cell fraction contains 3 to 4 times more C14 activity per milliliter than does the supernatant fluid when the isotope is fed. In the supernatant fluid the activity is all within precipitable protein, much of which is probably derived from the blood plasma. In spite of increased loss of C14 as CO2 in the expired air and in the pus, there is evidence of conservation of protein-building materials for maintenance of new plasma proteins and tissue proteins in the more active organs (e.g. liver)—a shift of protein C14 from the less active tissues (muscle and skin).
Full Text
The Full Text of this article is available as a PDF (1.1 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Gibson J. G., Evelyn K. A. CLINICAL STUDIES OF THE BLOOD VOLUME. IV. ADAPTATION OF THE METHOD TO THE PHOTOELECTRIC MICROCOLORIMETER. J Clin Invest. 1938 Mar;17(2):153–158. doi: 10.1172/JCI100938. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Madden S. C., Finch C. A., Swalbach W. G., Whipple G. H. BLOOD PLASMA PROTEIN PRODUCTION AND UTILIZATION : THE INFLUENCE OF AMINO ACIDS AND OF STERILE ABSCESSES. J Exp Med. 1940 Feb 29;71(3):283–297. doi: 10.1084/jem.71.3.283. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Madden S. C., Winslow P. M., Rowland J. W., Whipple G. H. BLOOD PLASMA PROTEIN REGENERATION AS INFLUENCED BY INFECTION, DIGESTIVE DISTURBANCES, THYROID, AND FOOD PROTEINS : A DEFICIENCY STATE RELATED TO PROTEIN DEPLETION. J Exp Med. 1937 Feb 28;65(3):431–454. doi: 10.1084/jem.65.3.431. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McNaught J. B., Scott V. C., Woods F. M., Whipple G. H. BLOOD PLASMA PROTEIN REGENERATION CONTROLLED BY DIET : EFFECTS OF PLANT PROTEINS COMPARED WITH ANIMAL PROTEINS THE INFLUENCE OF FASTING AND INFECTION. J Exp Med. 1936 Jan 31;63(2):277–301. doi: 10.1084/jem.63.2.277. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robscheit-Robbins F. S., Whipple G. H. INFECTION AND INTOXICATION : THEIR INFLUENCE UPON HEMOGLOBIN PRODUCTION IN EXPERIMENTAL ANEMIA. J Exp Med. 1936 Apr 30;63(5):767–787. doi: 10.1084/jem.63.5.767. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STERLING K. The turnover rate of serum albumin in man as measured by I131-tagged albumin. J Clin Invest. 1951 Nov;30(11):1228–1237. doi: 10.1172/JCI102542. [DOI] [PMC free article] [PubMed] [Google Scholar]
- YUILE C. L., LAMSON B. G., MILLER L. L., WHIPPLE G. H. Conversion of plasma protein to tissue protein without evidence of protein breakdown; results of giving plasma protein labeled with carbon 14 parenterally to dogs. J Exp Med. 1951 Jun;93(6):539–557. doi: 10.1084/jem.93.6.539. [DOI] [PMC free article] [PubMed] [Google Scholar]
- YUILE C. L., O'DEA A. E., LUCAS F. V., WHIPPLE G. H. Plasma protein labeled with lysine-epsilon-C14; its oral feeding and related protein metabolism in the dog. J Exp Med. 1952 Sep;96(3):247–254. doi: 10.1084/jem.96.3.247. [DOI] [PMC free article] [PubMed] [Google Scholar]