Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1953 Aug 1;98(2):107–117. doi: 10.1084/jem.98.2.107

OBSERVATIONS CONCERNING THE PRODUCTION AND EXCRETION OF CHOLESTEROL IN MAMMALS

X. FACTORS AFFECTING THE ABSORPTION AND FATE OF INGESTED CHOLESTEROL

Meyer Friedman 1, Sanford O Byers 1, Eichi Shibata 1
PMCID: PMC2136282  PMID: 13069654

Abstract

The absorption of oral cholesterol by rats was followed directly by analyses of the thoracic lymph and indirectly by calculating the deficit of fecal cholesterol under the amount fed. The two methods checked within about 20 per cent, with fecal analyses indicating the greater absorption. The absorbed cholesterol was found to be deposited in the liver, only very minor quantities being found in other organs or plasma. Bile is necessary for normal absorption of cholesterol, although very small amounts are absorbed even in its absence. Excess oral cholic acid increases the absorption of cholesterol by otherwise normal animals. The rat absorbs about 47 per cent of a single 50 mg. dose of cholesterol and about 34 per cent of a 100 mg. dose.

Full Text

The Full Text of this article is available as a PDF (584.3 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BIGGS M. W., FRIEDMAN M., BYERS S. O. Intestinal lymphatic transport of absorbed cholesterol. Proc Soc Exp Biol Med. 1951 Nov;78(2):641–643. doi: 10.3181/00379727-78-19167. [DOI] [PubMed] [Google Scholar]
  2. BLOOM B., CHAIKOFF I. L., REINHARDT W. O., ENTENMAN C., DAUBEN W. G. The quantitative significance of the lymphatic pathway in transport of absorbed fatty acids. J Biol Chem. 1950 May;184(1):1–8. [PubMed] [Google Scholar]
  3. BYERS S. O., BIGGS M. W. Cholic acid and cholesterol: studies concerning possible intraconversion. Arch Biochem Biophys. 1952 Aug;39(2):301–304. doi: 10.1016/0003-9861(52)90339-1. [DOI] [PubMed] [Google Scholar]
  4. BYERS S. O., FRIEDMAN M., BIGGS M. W., GUNNING B. Observations concerning the production and excretion of cholesterol in mammals. IX. The mechanism of the hypercholesteremic effect of cholic acid. J Exp Med. 1953 Apr;97(4):511–524. doi: 10.1084/jem.97.4.511. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. BYERS S. O., FRIEDMAN M. Effect of various bile acids on the hypercholesteremia following biliary obstruction in the rat. Am J Physiol. 1952 Jan;168(1):138–139. doi: 10.1152/ajplegacy.1951.168.1.138. [DOI] [PubMed] [Google Scholar]
  6. BYERS S. O., FRIEDMAN M., MICHAELIS F. Observations concerning the production and excretion of cholesterol in mammals. III. The source of excess plasma cholesterol after ligation of the bile duct. J Biol Chem. 1951 Feb;188(2):637–641. [PubMed] [Google Scholar]
  7. BYERS S. O., FRIEDMAN M. Production and excretion of cholesterol in mammals. VII. Biliary cholesterol; increment and indicator of hepatic synthesis of cholesterol. Am J Physiol. 1952 Feb;168(2):297–302. doi: 10.1152/ajplegacy.1952.168.2.297. [DOI] [PubMed] [Google Scholar]
  8. BYERS S. O., ROSENMAN R. H., FRIEDMAN M., BIGGS M. W. Rate of cholesterol synthesis in hypo- and hyperthyroid rats. J Exp Med. 1952 Nov;96(5):513–516. doi: 10.1084/jem.96.5.513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. FLEISCHMANN W., STUBBS J. L., McSHANE W. P. Effect of alpha naphthylthiourea on serum cholesterol in thyroidectomized dogs. Proc Soc Exp Biol Med. 1949 Feb;70(2):246–248. doi: 10.3181/00379727-70-16889. [DOI] [PubMed] [Google Scholar]
  10. FRIEDMAN M., BYERS S. O. Cholic acid; an adequate stimulus for hypercholesteremia in the normal fasting rat. Proc Soc Exp Biol Med. 1951 Nov;78(2):528–529. doi: 10.3181/00379727-78-19128. [DOI] [PubMed] [Google Scholar]
  11. FRIEDMAN M., BYERS S. O., GUNNING B. Observations concerning production and excretion of cholesterol in mammals. VIII. Fate of injected cholesterol in the animal body. Am J Physiol. 1953 Feb;172(2):309–316. doi: 10.1152/ajplegacy.1953.172.2.309. [DOI] [PubMed] [Google Scholar]
  12. FRIEDMAN M., BYERS S. O., MICHAELIS F. Observations concerning production and excretion of cholesterol in mammals. II. Excretion of bile in the rat. Am J Physiol. 1950 Sep;162(3):575–578. doi: 10.1152/ajplegacy.1950.162.3.575. [DOI] [PubMed] [Google Scholar]
  13. FRIEDMAN M., BYERS S. O., MICHAELIS F. Production and excretion of cholesterol in mammals. Iv. Role of liver in restoration of plasma cholesterol after experimentally induced hypocholesteremia. Am J Physiol. 1951 Mar;164(3):789–791. doi: 10.1152/ajplegacy.1951.164.3.789. [DOI] [PubMed] [Google Scholar]
  14. FRIEDMAN M., BYERS S. O., ROSENMAN R. H. Changes in excretion of intestinal cholesterol and sterol digitonides in hyper and hypothyroidism. Circulation. 1952 May;5(5):657–660. doi: 10.1161/01.cir.5.5.657. [DOI] [PubMed] [Google Scholar]
  15. FRIEDMAN M., BYERS S. O. The mechanism responsible for the hypercholesteremia induced by triton WR-1339. J Exp Med. 1953 Jan;97(1):117–130. doi: 10.1084/jem.97.1.117. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. GOFMAN J. W., JONES H. B., LYON T. P., LINDGREN F., STRISOWER B., COLMAN D., HERRING V. Blood lipids and human atherosclerosis. Circulation. 1952 Jan;5(1):119–134. doi: 10.1161/01.cir.5.1.119. [DOI] [PubMed] [Google Scholar]
  17. KIM K. S., IVY A. C. Factors influencing cholesterol absorption. Am J Physiol. 1952 Nov;171(2):302–318. doi: 10.1152/ajplegacy.1952.171.2.302. [DOI] [PubMed] [Google Scholar]
  18. PAGE I. H., BROWN H. B. Induced hypercholesterolemia and atherogenesis. Circulation. 1952 Nov;6(5):681–687. doi: 10.1161/01.cir.6.5.681. [DOI] [PubMed] [Google Scholar]
  19. PAGE I. H., LEWIS L. A., PLAHL G. The lipoprotein composition of dog lymph. Circ Res. 1953 Jan;1(1):87–93. doi: 10.1161/01.res.1.1.87. [DOI] [PubMed] [Google Scholar]
  20. ROSENMAN R. H., FRIEDMAN M., BYERS S. O. Changes in biliary cholesterol abnormal thyroid states. Science. 1951 Aug 24;114(2956):210–211. doi: 10.1126/science.114.2956.210. [DOI] [PubMed] [Google Scholar]
  21. ROSENMAN R. H., SHIBATA E. Effect of age upon hepatic synthesis of cholesterol in rat. Proc Soc Exp Biol Med. 1952 Oct;81(1):296–298. doi: 10.3181/00379727-81-19855. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES