Abstract
The effect on cholesterol synthesis of ligation of the common bile duct was studied in the rat. The bile ducts of rats were ligated; 24 to 48 hours later, estimates of the rate of cholesterol synthesis were made, either by injection of labelled water or acetate into the intact animal, or by incubation of slices or homogenates of the liver in the presence of 1-C14-acetate. These various criteria all indicated that cholesterol synthesis was increased following ligation of the bile duct. The average ratios of the rate of synthesis in the experimental animals to that in the controls were as follows: 1. Synthesis from C14-carboxyl-labelled acetate: (a) in the intact rat fed ad libitum, 19; (b) in liver slices from the fasted rat, 23; (c) in liver slices from the rat fed ad libitum, 4; (d) in cell-free homogenates from the fasted rat, >27; (e) in cell-free homogenates from the rat fed ad libitum, 17. 2. Synthesis from tritium-labelled water in the intact rat fed ad libitum, 4.
Full Text
The Full Text of this article is available as a PDF (570.1 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BYERS S. O., FRIEDMAN M., MICHAELIS F. Observations concerning the production and excretion of cholesterol in mammals. III. The source of excess plasma cholesterol after ligation of the bile duct. J Biol Chem. 1951 Feb;188(2):637–641. [PubMed] [Google Scholar]
- BYERS S. O., FRIEDMAN M. Production and excretion of cholesterol in mammals. VII. Biliary cholesterol; increment and indicator of hepatic synthesis of cholesterol. Am J Physiol. 1952 Feb;168(2):297–302. doi: 10.1152/ajplegacy.1952.168.2.297. [DOI] [PubMed] [Google Scholar]
- BYERS S. O., FRIEDMAN M., ROSENMAN R. H. Review, on the regulation of blood cholesterol. Metabolism. 1952 Nov;1(6):479–503. [PubMed] [Google Scholar]
- FRIEDMAN M., BYERS S. Production and excretion of cholesterol in mammals. VI. Bile acid accumulation in production of hypercholesteremia occurring after biliary obstruction. Am J Physiol. 1952 Feb;168(2):292–296. doi: 10.1152/ajplegacy.1952.168.2.292. [DOI] [PubMed] [Google Scholar]
- SCHWENK E., WERTHESSEN N. T. Studies on the biosynthesis of cholesterol. III. Purification of C14-cholesterol from perfusions of livers and other organs. Arch Biochem Biophys. 1952 Oct;40(2):334–341. doi: 10.1016/0003-9861(52)90119-7. [DOI] [PubMed] [Google Scholar]
- SPERRY W. M., WEBB M. A revision of the Schoenheimer-Sperry method for cholesterol determination. J Biol Chem. 1950 Nov;187(1):97–106. [PubMed] [Google Scholar]
- TOMKINS G. M., CHAIKOFF I. L. Cholesterol synthesis by liver. I. Influence of fasting and of diet. J Biol Chem. 1952 May;196(2):569–573. [PubMed] [Google Scholar]
- VAN BRUGGEN J. T., HUTCHENS T. T., CLAYCOMB C. K., CATHEY W. J., WEST E. S. The effect of fasting upon lipogenesis in the intact rat. J Biol Chem. 1952 May;196(1):389–394. [PubMed] [Google Scholar]
- Wesson L. G. A MODIFICATION OF THE OSBORNEMENDEL SALT MIXTURE CONTAINING ONLY INORGANIC CONSTITUENTS. Science. 1932 Mar 25;75(1943):339–340. doi: 10.1126/science.75.1943.339. [DOI] [PubMed] [Google Scholar]