Abstract
A mucopolysaccharide inhibitor of Theiler's GDVII virus has been purified from intestinal tissue of adult mice. The purified material has been shown to consist chiefly of carbohydrate. Following acid hydrolysis of the inhibitor, several amino adds were found to be present. The carbohydrate components shown to be present thus far are galactose, hexosamine, N-acetylhexosamine, methylpentose, hexurornic acid; an indication has been obtained that sedoheptulose is also present. The available evidence strongly suggests that this mucopolysaccharide, and not some other substance present in minute amounts, is the inhibitor of the GDVII virus.
Full Text
The Full Text of this article is available as a PDF (477.9 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ANNISON E. F., MORGAN W. T. J. Studies in immunochemistry. X. The isolation and properties of Lewis (Lea) human blood group substance. Biochem J. 1952 Feb;50(4):460–471. doi: 10.1042/bj0500460. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bendich A., Kabat E. A., Bezer A. E., With an Appendix by Erwin Brand, Ph.D., and Leo J. Saidel IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : III. PROPERTIES OF PURIFIED BLOOD GROUP A SUBSTANCES FROM INDIVIDUAL HOG STOMACH LININGS. J Exp Med. 1946 May 31;83(6):485–497. [PMC free article] [PubMed] [Google Scholar]
- Consden R., Gordon A. H., Martin A. J. Qualitative analysis of proteins: a partition chromatographic method using paper. Biochem J. 1944;38(3):224–232. doi: 10.1042/bj0380224. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DISCHE Z., SHETTLES L. B., OSNOS M. New specific color reactions of hexoses and spectrophotometric micromethods for their determination. Arch Biochem. 1949 Jun;22(2):169–184. [PubMed] [Google Scholar]
- HIRST G. K. The nature of the virus receptors of red cells; evidence on the chemical nature of the virus receptors of red cells and of the existence of a closely analogous substance in normal serum. J Exp Med. 1948 Apr 1;87(4):301–314. doi: 10.1084/jem.87.4.301. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JESAITIS M. A., GOEBEL W. F. The chemical and antiviral properties of the somatic antigen of Phase II Shigella sonnei. J Exp Med. 1952 Nov;96(5):409–424. doi: 10.1084/jem.96.5.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- MANDEL B., RACKER E. Inhibition of Theiler's encephalomyelitis virus (GDVII strain) of mice by an intestinal mucopolysaccharide. I. Biological properties and mechanism of action. J Exp Med. 1953 Nov;98(5):399–415. doi: 10.1084/jem.98.5.399. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morgan W. T., Elson L. A. A colorimetric method for the determination of N-acetylglucosamine and N-acetylchrondrosamine. Biochem J. 1934;28(3):988–995. doi: 10.1042/bj0280988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morgan W. T., King H. K. Studies in immunochemistry: 7. The isolation from hog gastric mucin of the polysaccharide-amino acid complex possessing blood group A specificity. Biochem J. 1943;37(5):640–651. doi: 10.1042/bj0370640. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morris D. L. Quantitative Determination of Carbohydrates With Dreywood's Anthrone Reagent. Science. 1948 Mar 5;107(2775):254–255. doi: 10.1126/science.107.2775.254. [DOI] [PubMed] [Google Scholar]
- TAMM I., HORSFALL F. L., Jr A mucoprotein derived from human urine which reacts with influenza, mumps, and Newcastle disease viruses. J Exp Med. 1952 Jan;95(1):71–97. doi: 10.1084/jem.95.1.71. [DOI] [PMC free article] [PubMed] [Google Scholar]