Abstract
Tuberculin (P.P.D.) and cortisone acetate, singly and in combination, were added to explanted splenic tissue derived from guinea pigs, mice, and rabbits, and buffy coats obtained from rabbits. These tissues came from normal animals or from animals which had been infected with the tubercle bacillus or immunized with killed tubercle bacilli. 5 µg./ml. of tuberculin or 0.5 µg./ml. of cortisone was the largest concentration of the respective reagents which was tolerated by cultured cells from normal animals. From the results with all tissues studied it would appear that P.P.D. selectively damaged only the growing cells of splenic tissues from sensitive guinea pigs and to a lesser degree the migrating cells of buffy coats obtained from sensitive rabbits. Cortisone appeared to have increased toxicity for only explanted splenic cells from tuberculin-sensitive guinea pigs. The specific effect of P.P.D. on tissues from tuberculin-sensitive animals was not modified by cortisone under the conditions of these experiments. Thus these data furnished no evidence that cortisone had any direct effect on the response of the sensitive cells to P.P.D.
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Selected References
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- BALDRIDGE G. D., KLIGMAN A. M., LIPNIK M. J., PILLSBURY D. M. In vitro effects of cortisone on mesodermal tissue. AMA Arch Pathol. 1951 Jun;51(6):593–596. [PubMed] [Google Scholar]
- BUCKLEY J. J., BUCKLEY S. M., KEEVE M. I. Tissue culture studies on liver cells of tuberculin-sensitized animals in the presence of tuberculin (purified protein derivative). Bull Johns Hopkins Hosp. 1951 Oct;89(4):303–308. [PubMed] [Google Scholar]
- CORNMAN I. Selective damage to fibroblasts by desoxycorticosterone in cultures of mixed tissues. Science. 1951 Jan 12;113(2924):37–39. doi: 10.1126/science.113.2924.37. [DOI] [PubMed] [Google Scholar]
- LEAHY R. H., MORGAN H. R. The inhibition by cortisone of the cytotoxic activity of PPD on tuberculin-hypersensitive cells in tissue culture. J Exp Med. 1952 Dec;96(6):549–554. doi: 10.1084/jem.96.6.549. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PAFF G. H., STEWART R. Free wandering cells and cortisone. Proc Soc Exp Biol Med. 1953 Jul;83(3):591–592. doi: 10.3181/00379727-83-20428. [DOI] [PubMed] [Google Scholar]
- RUSKIN B., POMERAT C. M., RUSKIN A. Toxicity of various cortisone preparations on embryonic chick heart, spleen, and spinal cord in tissue culture. Tex Rep Biol Med. 1951;9(4):786–795. [PubMed] [Google Scholar]
- SCHREK R. Color test to measure the toxicity of adrenal cortex hormones to lymphocytes. Proc Soc Exp Biol Med. 1951 Mar;76(3):557–559. doi: 10.3181/00379727-76-18555. [DOI] [PubMed] [Google Scholar]