Abstract
A group of mutant phages stemming from the virus of B. megatherium 899a (lysogenic), growing on a sensitive B. megatherium strain (KM), have been studied with respect to their recombination reactions. All these mutants and many of their recombinations can be recognized by a characteristic plaque morphology. A similar group of phages have been isolated directly from a culture of B. megatherium 899a in this laboratory. Previous work has shown that when two different plaque mutant phages both infect essentially all the bacteria in a culture, a characteristic per cent of recombinants is produced. This percentage depends on the two recombinants used, each pair having its own value. Hershey and coworkers (2–5) have demonstrated with coli-phage T2, that the percentages of recombination found can be handled mathematically and that they demonstrate the existence of a relationship between the mutations entirely comparable to crossover percentages as used in gene locus maps in genetics. This has been found to hold true for the phages studied in the present work. Only one "linkage group" has been detected and all the mutants studied showed low percentages of recombination (0.8 to 7.6). B. megatherium 899a phage and some of its mutants have been examined with an electron microscope and no differences have been detected between the different mutant strains.
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Selected References
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- BURNET F. M. Some biological implications of studies on influenza viruses. Bull Johns Hopkins Hosp. 1951 Feb;88(2):119–180. [PubMed] [Google Scholar]
- Doermann A H, Hill M B. Genetic Structure of Bacteriophage T4 as Described by Recombination Studies of Factors Influencing Plaque Morphology. Genetics. 1953 Jan;38(1):79–90. doi: 10.1093/genetics/38.1.79. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GRAZIOSI F., RUSSO G. Studio al microscopio elettronico di un batteriofago attivo sul B. megatherium. Rend Ist Sup Sanit. 1951;14(10):839–844. [PubMed] [Google Scholar]
- HERSHEY A. D., CHASE M. Genetic recombination and heterozygosis in bacteriophage. Cold Spring Harb Symp Quant Biol. 1951;16:471–479. doi: 10.1101/sqb.1951.016.01.034. [DOI] [PubMed] [Google Scholar]
- HIRST G. K., GOTLIEB T. The experimental production of combination forms of virus. I. Occurrence of combination forms after simultaneous inoculation of the allantoic sac with two distinct strains of influenza virus. J Exp Med. 1953 Jul;98(1):41–52. doi: 10.1084/jem.98.1.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hershey A D, Davidson H. Allelic and Nonallelic Genes Controlling Host Specificity in a Bacteriophage. Genetics. 1951 Nov;36(6):667–675. doi: 10.1093/genetics/36.6.667. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MURPHY J. S. Mutants of a bacteriophage of Bacillus megatherium. J Exp Med. 1952 Dec;96(6):581–588. doi: 10.1084/jem.96.6.581. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NORTHROP J. H. Growth and phage production of lysogenic B. megatherium. J Gen Physiol. 1951 May;34(5):715–735. doi: 10.1085/jgp.34.5.715. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Visconti N, Delbrück M. The Mechanism of Genetic Recombination in Phage. Genetics. 1953 Jan;38(1):5–33. doi: 10.1093/genetics/38.1.5. [DOI] [PMC free article] [PubMed] [Google Scholar]