Abstract
The period and rate of liberation of influenza virus from entodermal cells of the allantois have been studied by deembryonating eggs within a few minutes after infection, exchanging the medium thereafter at hourly intervals and assaying the virus concentration in the harvests thus obtained (differential growth curves). If the inoculum was sufficiently large, presumably all available cells immediately became infected and only 1 infectious cycle was expected to occur. If the inoculum was small, so that only a fraction of the cells adsorbed virus, the infectious process was held to 1 cycle by continuous exposure of the remaining susceptible cells to RDE. In either case, the results obtained indicate that once cells have been infected they produce and liberate virus at nearly constant rates for periods of 30 hours or longer before the yields decrease rapidly. Evidence has been presented which strongly suggests that such prolonged periods of liberation are observed not only in deembryonated eggs but also in the intact chick embryo. Attempts have been made in the discussion to reconcile these findings with previous estimates of the liberation period and to integrate them with histologic observations and electron micrographs of thin sections of infected allantoic membranes having a bearing on the mode of liberation.
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Selected References
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- CHU C. M., DAWSON I. M., ELFORD W. J. Filamentous forms associated with newly isolated influenza virus. Lancet. 1949 Apr 9;1(6554):602–602. doi: 10.1016/s0140-6736(49)91699-2. [DOI] [PubMed] [Google Scholar]
- DULBECCO R., VOGT M. One-step growth curve of Western equine encephalomyelitis virus on chicken embryo cells grown in vitro and analysis of virus yields from single cells. J Exp Med. 1954 Feb;99(2):183–199. doi: 10.1084/jem.99.2.183. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EDDY B. E., WYCKOFF R. W. G. Influenza virus in sectioned tissues. Proc Soc Exp Biol Med. 1950 Oct;75(1):290–293. doi: 10.3181/00379727-75-18174. [DOI] [PubMed] [Google Scholar]
- Henle W., Henle G., Rosenberg E. B. THE DEMONSTRATION OF ONE-STEP GROWTH CURVES OF INFLUENZA VIRUSES THROUGH THE BLOCKING EFFECT OF IRRADIATED VIRUS ON FURTHER INFECTION. J Exp Med. 1947 Oct 31;86(5):423–437. doi: 10.1084/jem.86.5.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ISAACS A., EDNEY M. Interference between inactive and active influenza viruses in the chick embryo. Aust J Exp Biol Med Sci. 1950 Nov;28(6):635–645. doi: 10.1038/icb.1950.66. [DOI] [PubMed] [Google Scholar]
- Knight C. A. TITRATION OF INFLUENZA VIRUS IN CHICK EMBRYOS. J Exp Med. 1944 May 1;79(5):487–495. doi: 10.1084/jem.79.5.487. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MURPHY J. S., BANG F. B. Observations with the electron microscope on cells of the chick chorio-allantoic membrane infected with influenza virus. J Exp Med. 1952 Mar;95(3):259–268. doi: 10.1084/jem.95.3.259. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MURPHY J. S., KARZON D. T., BANG F. B. Studies of influenza A (pr8) infected tissue cultures by electron microscopy. Proc Soc Exp Biol Med. 1950 Apr;73(4):596–599. doi: 10.3181/00379727-73-17756. [DOI] [PubMed] [Google Scholar]
- WYCKOFF R. W. G. Formation of the particles of influenza virus. J Immunol. 1953 Feb;70(2):187–196. [PubMed] [Google Scholar]
- von MAGNUS P. Propagation of the PR8 strain of influenza A virus in chick embryos. II. The formation of incomplete virus following inoculation of large doses of seed virus. Acta Pathol Microbiol Scand. 1951;28(3):278–293. doi: 10.1111/j.1699-0463.1951.tb03693.x. [DOI] [PubMed] [Google Scholar]