Abstract
A vacuum sublimation apparatus is described which will permit, (a) the removal of water from virus suspensions at temperatures ranging down to –80°C., (b) continuous operation with a minimum of attention from the investigator, (c) sealing off of samples at operating pressures (10–5 mm. Hg), (d) simultaneous lyophilization of aliquot samples at different temperatures, (e) isolation of a portion of the apparatus without disturbing the remainder of the system, and (f) determination of the end-point of sublimation without disturbing the samples. The time required for drying 0.1 ml. of influenza virus suspension was shown to increase markedly with decrease of temperature, 8 days being required for dehydration at –80°C. in contrast to 2 days at –30°C. and 1 day at 0°C.
Full Text
The Full Text of this article is available as a PDF (430.7 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bauer J. H., Pickels E. G. APPARATUS FOR FREEZING AND DRYING VIRUS IN LARGE QUANTITIES UNDER UNIFORM CONDITIONS. J Exp Med. 1940 Jan 1;71(1):83–88. doi: 10.1084/jem.71.1.83. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HUTTON R. S., HILMOE R. J., ROBERTS J. L. Some physical factors that influence the survival of Brucella abortus during freeze-drying. J Bacteriol. 1951 Mar;61(3):309–319. doi: 10.1128/jb.61.3.309-319.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Naylor H. B., Smith P. A. Factors Affecting the Viability of Serratia marcescens During Dehydration and Storage. J Bacteriol. 1946 Nov;52(5):565–573. doi: 10.1128/jb.52.5.565-573.1946. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PROOM H., HEMMONS L. M. The drying and preservation of bacterial cultures. J Gen Microbiol. 1949 Jan;3(1):7–18. doi: 10.1099/00221287-3-1-7. [DOI] [PubMed] [Google Scholar]