Abstract
A method is described for obtaining crystalline C-reactive protein from serous fluids in which the protein is associated with lipid. Most pathological fluids currently available as a source of this protein appear to fall in this category. Crystalline C-reactive protein has its isoelectric point at pH 4.82 as determined by free electrophoresis in McIlvaine's buffer. Its mobility in the electrophoresis cell, both alone and after addition to normal serum, coincides with that of the β-globulin fraction of the serum. In contrast to this finding, by the method of zone electrophoresis on a starch supporting medium the protein migrates with the γ1-globulin. The significance of this discrepancy is discussed. Studies in the ultracentrifuge indicate an s 20,w of 7.5.
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Selected References
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- KUNKEL H. G., SLATER R. J. Zone electrophoresis in a starch supporting medium. Proc Soc Exp Biol Med. 1952 May;80(1):42–44. doi: 10.3181/00379727-80-19516. [DOI] [PubMed] [Google Scholar]
- Macleod C. M., Avery O. T. THE OCCURRENCE DURING ACUTE INFECTIONS OF A PROTEIN NOT NORMALLY PRESENT IN THE BLOOD : II. ISOLATION AND PROPERTIES OF THE REACTIVE PROTEIN. J Exp Med. 1941 Jan 31;73(2):183–190. doi: 10.1084/jem.73.2.183. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Macleod C. M., Avery O. T. THE OCCURRENCE DURING ACUTE INFECTIONS OF A PROTEIN NOT NORMALLY PRESENT IN THE BLOOD : III. IMMUNOLOGICAL PROPERTIES OF THE C-REACTIVE PROTEIN AND ITS DIFFERENTIATION FROM NORMAL BLOOD PROTEINS. J Exp Med. 1941 Jan 31;73(2):191–200. doi: 10.1084/jem.73.2.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McCarty M. THE OCCURRENCE DURING ACUTE INFECTIONS OF A PROTEIN NOT NORMALLY PRESENT IN THE BLOOD : IV. CRYSTALLIZATION OF THE C-REACTIVE PROTEIN. J Exp Med. 1947 Apr 30;85(5):491–498. doi: 10.1084/jem.85.5.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WOOD H. F., McCARTY M. The measurement of C-reactive protein in human sera; comparison of the clinical tests on the basis of a quantitative method. J Clin Invest. 1951 Jun;30(6):616–622. doi: 10.1172/JCI102479. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WOOD H. F. The relationship between the acute phase response and antibody production in the rabbit. I. Correlation between the early appearance of Cx-reaction protein and subsequent antibody production. J Exp Med. 1953 Oct;98(4):311–319. doi: 10.1084/jem.98.4.311. [DOI] [PMC free article] [PubMed] [Google Scholar]