Abstract
The development of viral neutralizing antibodies in animals injected with T3 or T4 phage is considerably inhibited by the presence of bacterial antigens. A new procedure has been described to liberate phage from infected E. coli B bacteria by inducing lysis with penicillin. By immunological means it has been shown that T4-infected cultures of E. coli B, in which phage development has been inhibited with proflavine, contain the viral neutralizing antigen after lysis. In contrast, it has not been possible to demonstrate by immunological means the appearance of viral neutralizing antigen in E. coli B infected with T3 prior to the appearance of intracellular phage.
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Selected References
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- BARRY G. T., GOEBEL W. F. The effect of chemical and physical agents on the phage receptor of Phase II Shigella sonnei. J Exp Med. 1951 Nov;94(5):387–400. doi: 10.1084/jem.94.5.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BENZER S. Resistance to ultraviolet light as an index to the reproduction of bacteriophage. J Bacteriol. 1952 Jan;63(1):59–72. doi: 10.1128/jb.63.1.59-72.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DE MARS R. I., LURIA S. E., FISHER H., LEVINTHAL C. The production of incomplete bacteriophage particles by the action of proflavine and the properties of the incomplete particles. Ann Inst Pasteur (Paris) 1953 Jan;84(1):113–128. [PubMed] [Google Scholar]
- DOERMANN A. H. The intracellular growth of bacteriophages. I. Liberation of intracellular bacteriophage T4 by premature lysis with another phage or with cyanide. J Gen Physiol. 1952 Mar;35(4):645–656. doi: 10.1085/jgp.35.4.645. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Foster R. A. An Analysis of the Action of Proflavine on Bacteriophage Growth. J Bacteriol. 1948 Dec;56(6):795–809. doi: 10.1128/jb.56.6.795-809.1948. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HERSHEY A. D., CHASE M. Independent functions of viral protein and nucleic acid in growth of bacteriophage. J Gen Physiol. 1952 May;36(1):39–56. doi: 10.1085/jgp.36.1.39. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROUNTREE P. M. A complement-fixing antigen of bact. coli bacteriophage T5: its behaviour during virus growth. Br J Exp Pathol. 1951 Aug;32(4):341–352. [PMC free article] [PubMed] [Google Scholar]