Abstract
Vaccinia and fowl pox viruses were visualized by the electron microscope in sections of infected chorioallantoic membrane of chicken embryos. The viruses were of similar structure and size, averaging 200 x 300 mµ with considerable individual variation. Intracytoplasmic viral particles contained a dense, nucleus-like body (nucleoid) separated from granular material (viroplasm) by a zone of lesser density. They were enclosed by a single membrane. Near the surface of the host cell and in the extracellular space the particles consisted of a central body of variable shape and density enclosed by a double membrane. The initial sites of development were confined to the cytoplasm of the host cell. Before release from the host cell the viral nucleoids appeared to enlarge and to occupy a central position within the particle, which became enclosed by a double limiting membrane. The brick-shaped forms found after removal of the embedding plastic from thick sections indicated that drying caused characteristic distortion of certain viral particles.
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Selected References
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- BANG F. B. Cellular changes in the chick chorio-allantoic membrane infected with herpes simplex and vaccinia; a study with thin sections for the electron microscope. Bull Johns Hopkins Hosp. 1950 Dec;87(6):511–547. [PubMed] [Google Scholar]
- BANG F. B., LEVY E., GEY G. O. Some observations on host-cell-virus relationships in fowl pox. I. Growth in tissue culture. II. The inclusion produced by the virus on the chick chorio-allantoic membrane. J Immunol. 1951 Mar;66(3):329–345. [PubMed] [Google Scholar]
- FARRANT J. L., FENNER F. A comparison of the morphology of vaccinia and myxoma viruses. Aust J Exp Biol Med Sci. 1953 Apr;31(2):121–125. doi: 10.1038/icb.1953.14. [DOI] [PubMed] [Google Scholar]
- GAYLORD W. H., Jr, MELNICK J. L. Intracellular forms of pox viruses as shown by the electron microscope (Vaccinia, Ectromelia, Molluscum Contagiosum). J Exp Med. 1953 Aug;98(2):157–172. doi: 10.1084/jem.98.2.157. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Green R. H., Anderson T. F., Smadel J. E. MORPHOLOGICAL STRUCTURE OF THE VIRUS OF VACCINIA. J Exp Med. 1942 Jun 1;75(6):651–656. doi: 10.1084/jem.75.6.651. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MORGAN C., ELLISON S. A., ROSE H. M., MOORE D. H. Internal structure in virus particles. Nature. 1954 Jan 30;173(4396):208–208. doi: 10.1038/173208a0. [DOI] [PubMed] [Google Scholar]
- MORGAN C., ELLISON S. A., ROSE H. M., MOORE D. H. Structure and development of viruses as observed in the electron microscope. I. Herpes simplex virus. J Exp Med. 1954 Aug 1;100(2):195–202. doi: 10.1084/jem.100.2.195. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MORGAN C., WYCKOFF R. W. G. The electron microscopy of fowl pox virus within the chorioallantoic membrane. J Immunol. 1950 Aug;65(2):285–295. [PubMed] [Google Scholar]
- PALADE G. E. An electron microscope study of the mitochondrial structure. J Histochem Cytochem. 1953 Jul;1(4):188–211. doi: 10.1177/1.4.188. [DOI] [PubMed] [Google Scholar]
- Woodruff A. M., Goodpasture E. W. The Susceptibility of the Chorio-Allantoic Membrane of Chick Embryos to Infection with the Fowl-Pox Virus. Am J Pathol. 1931 May;7(3):209–222.5. [PMC free article] [PubMed] [Google Scholar]
- Wyckoff R. W. The Virus of Vaccinia in Chick Embryo Membrane. Proc Natl Acad Sci U S A. 1951 Sep;37(9):565–569. doi: 10.1073/pnas.37.9.565. [DOI] [PMC free article] [PubMed] [Google Scholar]