Abstract
The growth cycle of the virus of herpes simplex in chick embryo liver has been shown to follow the same pattern as in the chorioallantoic membrane and the rabbit's corneal cells. However, there is considerable variability in the time taken for the yolk sac-inoculated virus to get from the yolk sac into the liver. A brief description has been given of various fractionation procedures employed for obtaining isolated nuclei. It has been shown that free virus is not selectively adsorbed to isolated nuclei. Evidence has been presented to show that in the herpes-infected chick embryo liver, large proportions of the total virus can at times be found associated with the nuclear fraction. The percentage of the total virus in the nuclear fraction varies inversely with the titer of virus in the whole liver, and the number of hours after inoculation of the virus; only a negligible amount (as compared with that in the total) being associated with the nuclear fraction when a period of over 12 hours has elapsed after reappearance of virus. Furthermore, demonstration of virus in the isolated nuclei following extraction with hypertonic NaCl provides additional evidence that this virus is intimately associated with the nuclei.
Full Text
The Full Text of this article is available as a PDF (615.6 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ACKERMANN W. W., KURTZ H. The relation of herpes virus to host cell mitochondria. J Exp Med. 1952 Aug;96(2):151–157. doi: 10.1084/jem.96.2.151. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CROUSE H. V., CORIELL L. L., BLANK H., SCOTT T. F. M. Cytochemical studies on the intranuclear inclusion of herpes simplex. J Immunol. 1950 Jul;65(1):119–128. [PubMed] [Google Scholar]
- FRANCIS T., Jr, KURTZ H. B. The relation of herpes virus to the cell nucleus. Yale J Biol Med. 1950 Jul;22(6):579–587. [PMC free article] [PubMed] [Google Scholar]
- HENLE W., LIU O. C., FINTER N. B. Studies on host-virus interactions in the chick embryo-influenza virus system. IX. The period of liberation of virus from infected cells. J Exp Med. 1954 Jul 1;100(1):53–70. doi: 10.1084/jem.100.1.53. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KAPLAN A. S., MELNICK J. L. The intracellular localization of poliomyelitis virus. J Exp Med. 1953 Jan;97(1):91–116. doi: 10.1084/jem.97.1.91. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MORGAN C., ELLISON S. A., ROSE H. M., MOORE D. H. Electron microscopic examination of inclusion bodies of herpes simplex virus. Proc Soc Exp Biol Med. 1953 Mar;82(3):454–457. [PubMed] [Google Scholar]
- MORGAN C., ELLISON S. A., ROSE H. M., MOORE D. H. Structure and development of viruses as observed in the electron microscope. I. Herpes simplex virus. J Exp Med. 1954 Aug 1;100(2):195–202. doi: 10.1084/jem.100.2.195. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SCOTT T. F. M., BURGOON C. F., CORIELL L. L., BLANK H. The growth curve of the virus of herpes simplex in rabbit corneal cells grown in tissue culture with parallel observations on the development of the intranuclear inclusion body. J Immunol. 1953 Dec;71(6):385–396. [PubMed] [Google Scholar]
- SCOTT T. F., CORIELL L. L., BLANK H., GRAY A. The growth curve of the virus of herpes simplex on the choriollantoic membrane of the embryonated hen's egg. J Immunol. 1953 Sep;71(3):134–144. [PubMed] [Google Scholar]
- WOLMAN M., BEHAR A. Cytochemical evidence for the nature of herpes simplex inclusion bodies. J Infect Dis. 1952 Jul-Aug;91(1):63–68. doi: 10.1093/infdis/91.1.63. [DOI] [PubMed] [Google Scholar]