Abstract
Addition of the specific antigen to slices of liver or lung taken from sensitized guinea pigs, or the addition of anaphylactoid agents (tween 20, octadecylamine, morphine, and 48/80) to tissue slices from normal animals, or the perfusion of lung with these agents, has been shown to cause protein breakdown and liberation of histamine and heparin. The dose correlation between these phenomena raises the question of which is the causal event. Suppression of histamine and heparin release by inhibition of proteolysis suggests that the latter is the more fundamental reaction, but the problem probably can not be decided on the basis of present knowledge. Tissue proteolysis induced by the agents investigated in this work results from the action of a protease present in normal tissues as an inactive precursor. Conversion of the proenzyme requires the intervention of a kinase. The tissue kinase seems to be different from the serum kinase which has been shown to be related to complement. Serum kinase, however, also acts on tissue proenzyme and probably plays an important role in tissue reactions as elicited in the intact animal.
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Selected References
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- ASTRUP T., STERNDORFF I. Fibrinolytic activity of tissue extracts and of trypsin. Nature. 1952 Dec 6;170(4336):981–981. doi: 10.1038/170981a0. [DOI] [PubMed] [Google Scholar]
- CODE C. F. Histamine in blood. Physiol Rev. 1952 Jan;32(1):47–65. doi: 10.1152/physrev.1952.32.1.47. [DOI] [PubMed] [Google Scholar]
- Jaques L. B., Waters E. T. The identity and origin of the anticoagulant of anaphylactic shock in the dog. J Physiol. 1941 Jun 30;99(4):454–466. doi: 10.1113/jphysiol.1941.sp003915. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KOCHOLATY W., ELLIS W. W., JENSEN H. Activation of plasminogen by trypsin and plasmin. Blood. 1952 Sep;7(9):882–890. [PubMed] [Google Scholar]
- MACINTOSH F. C., PATON W. D. M. The liberation of histamine by certain organic bases. J Physiol. 1949 Aug;109(1-2):190–219. doi: 10.1113/jphysiol.1949.sp004385. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MCINTIRE F. C., ROTH L. W., SPROULL M. Mechanism of anaphylaxis in the rabbit; further evidence against plasma protease mechanism. Proc Soc Exp Biol Med. 1952 Dec;81(3):691–692. doi: 10.3181/00379727-81-19989. [DOI] [PubMed] [Google Scholar]
- MONKHOUSE F. C., FIDLAR E., BARLOW J. C. D. Release of heparin in anaphylactic shock in irradiated and non-irradiated animals. Am J Physiol. 1952 Jun;169(3):712–720. doi: 10.1152/ajplegacy.1952.169.3.712. [DOI] [PubMed] [Google Scholar]
- MONKHOUSE F. C., JAQUES L. B. An improved method for the extraction of heparin from blood. J Lab Clin Med. 1950 Nov;36(5):782–789. [PubMed] [Google Scholar]
- McINTIRE F. C., ROTH L. W., SPROULL M. In vitro histamine release from sensitized rabbit blood cells; evidence against participation of fibrinolysin. Proc Soc Exp Biol Med. 1950 Apr;73(4):605–609. doi: 10.3181/00379727-73-17759. [DOI] [PubMed] [Google Scholar]
- PATON W. D. M. Compound 48/80: a potent histamine liberator. Br J Pharmacol Chemother. 1951 Sep;6(3):499–508. doi: 10.1111/j.1476-5381.1951.tb00661.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROCHA E SILVA M., ARONSON M. Histamine release from the perfused lung of the guinea pig by serotoxin, anaphylatoxin. Br J Exp Pathol. 1952 Dec;33(6):577–586. [PMC free article] [PubMed] [Google Scholar]
- Tagnon H. J., Palade G. E. ACTIVATION OF PROPLASMIN BY A FACTOR FROM MAMMALIAN TISSUE. J Clin Invest. 1950 Mar;29(3):317–324. doi: 10.1172/JCI102260. [DOI] [PMC free article] [PubMed] [Google Scholar]
- UNGAR G., DAMGAARD E., HUMMEL F. P. Action of salicylates and related drugs on inflammation. Am J Physiol. 1952 Dec;171(3):545–553. doi: 10.1152/ajplegacy.1952.171.3.545. [DOI] [PubMed] [Google Scholar]
- UNGAR G., DAMGAARD E., HUMMEL F. P. Activation of profibrinolysin by antigen-antibody reaction and by anaphylactoid agents; its relation to complement. J Exp Med. 1953 Oct;98(4):291–303. doi: 10.1084/jem.98.4.291. [DOI] [PMC free article] [PubMed] [Google Scholar]
- UNGAR G., DAMGAARD E., HUMMEL F. P. The fibrinolysin-antifibrinolysin system in serum; mechanism of its endocrine control. Endocrinology. 1951 Dec;49(6):805–816. doi: 10.1210/endo-49-6-805. [DOI] [PubMed] [Google Scholar]
- UNGAR G. Inflammation and its control; a biochemical approach. Lancet. 1952 Oct 18;2(6738):742–746. doi: 10.1016/s0140-6736(52)91233-6. [DOI] [PubMed] [Google Scholar]
- UNGAR G., MIST S. H. Observations on the release of serum fibrinolysin by specific antigen, peptone, and certain polysaccharides. J Exp Med. 1949 Jul;90(1):39–51. doi: 10.1084/jem.90.1.39. [DOI] [PMC free article] [PubMed] [Google Scholar]