Abstract
The activation of poliomyelitis virus from a "neutral" virus-antibody mixture by the addition of non-infectious virus is described. The phenomenon was demonstrated in tissue cultures using both Petri dishes and roller tubes. The reaction was found to be type-specific and a relationship between antigen dilution and number of plaques counted was established. The relative potency of vaccine preparations determined in this system was found to be comparable to their relative potency in respect to protection-inducing activity in mice.
Full Text
The Full Text of this article is available as a PDF (445.3 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- CASALS J., OLITSKY P. K., BROWN L. V. Immunization of mice with inactivated type 2 poliomyelitis virus against types 2 and 3 viruses. Proc Soc Exp Biol Med. 1952 Aug-Sep;80(4):731–736. doi: 10.3181/00379727-80-19745. [DOI] [PubMed] [Google Scholar]
- DULBECCO R., VOGT M. Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med. 1954 Feb;99(2):167–182. doi: 10.1084/jem.99.2.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HOWE H. A. Antibody response of chimpanzees and human beings to formalin-inactivated trivalent poliomyelitis vaccine. Am J Hyg. 1952 Nov;56(3):265–286. doi: 10.1093/oxfordjournals.aje.a119551. [DOI] [PubMed] [Google Scholar]
- SALK J. E., BAZELEY P. L., BENNETT B. L., KRECH U., LEWIS L. J., WARD E. N., YOUNGNER J. S. Studies in human subjects on active immunization against poliomyelitis. II. A practical means for inducing and maintaining antibody formation. Am J Public Health Nations Health. 1954 Aug;44(8):994–1009. doi: 10.2105/ajph.44.8.994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SALK J. E., KRECH U., YOUNGNER J. S., BENNETT B. L., LEWIS L. J., BAZELEY P. L. Formaldehyde treatment and safety testing of experimental poliomyelitis vaccines. Am J Public Health Nations Health. 1954 May;44(5):563–570. doi: 10.2105/ajph.44.5.563. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SALK J. E., LEWIS L. J., YOUNGNER J. S., BENNETT B. L. The use of adjuvants to facilitate studies on the immunologic classification of poliomyelitis viruses. Am J Hyg. 1951 Sep;54(2):157–173. doi: 10.1093/oxfordjournals.aje.a119474. [DOI] [PubMed] [Google Scholar]
- SCHWERDT C. E., DICK G. W. A., HERRIOTT R. M., HOWE H. A. Immunization of cotton rats with inactivated Lansing poliomyelitis virus. I. Inactivation by chemical agents. Am J Hyg. 1951 Mar;53(2):121–130. doi: 10.1093/oxfordjournals.aje.a119439. [DOI] [PubMed] [Google Scholar]
- WENNER H. A., MILLER C. A., KAMITSUKA P., WILSON J. C. Preparation and standardization of antiserums prepared with the three known types to poliomyelitis viruses. Am J Hyg. 1954 Mar;59(2):221–235. doi: 10.1093/oxfordjournals.aje.a119635. [DOI] [PubMed] [Google Scholar]